Transposition of the Drosophila element mariner into the chicken germ line

被引:110
作者
Sherman, A
Dawson, A
Mather, C
Gilhooley, H
Li, Y
Mitchell, R
Finnegan, D
Sang, H [1 ]
机构
[1] Roslin Inst, Div Dev & Reprod, Roslin EH25 9PS, Midlothian, Scotland
[2] Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland
基金
英国惠康基金;
关键词
transgenic animals; transposable element;
D O I
10.1038/3497
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The ability of the Drosophila transposable element mariner to transpose in the chicken was tested using a plasmid carrying an active mariner element injected into chick zygotes. Surviving embryos and chicks were analyzed for presence of mariner. Analysis of embryos that survived for at least 12 days of development indicated that mariner had transposed at high frequency into the chicken genome, Germline transmission of mariner from one of three surviving birds confirmed transposition. Analysis of the first-generation (G(1)) chicks showed that they each contained between one and three copies of mariner. Six different transposition events were represented in the G(1) birds, and the transposition was catalyzed by expression of the mariner element's transposase gene, Transmission from G(1) to G(2) occurred at a 1:1 ratio, Mariner therefore has potential for development as a vector for transgenesis in avian species.
引用
收藏
页码:1050 / 1053
页数:4
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