The influence of environmental conditions on polysaccharide formation by Ganoderma lucidum in submerged cultures

被引：154

作者：

Yang, FC ^{[1
]}

Liau, CB ^{[1
]}

机构：

[1] Tunghai Univ, Dept Chem Engn, Taichung 40704, Taiwan

来源：

PROCESS BIOCHEMISTRY | 1998年 / 33卷 / 05期

关键词：

Ganoderma lucidum; polysaccharide; submerged culture;

D O I：

10.1016/S0032-9592(98)00023-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of environmental parameters on polysaccharide formation by Ganoderma lucidum were investigated in submerged cultures. The optimal temperature and pH was 30-35 degrees C and 4-4.5, respectively, in a glucose-ammonium chloride medium and polysaccharide concentration reached 1.6 mg/ml. Agitation and aeration influenced the formation and secretion of polysaccharide. The optimal rotating speed was 150 rpm in 7-day flask cultures, while the agitation speed employed in fermenter culture greatly affected the production rate and maximum concentration of polysaccharide. Although higher speeds enhanced mixing efficiency and polysaccharide release, higher shear stress had a detrimental effect on mycelial growth and polysaccharide formation. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：547 / 553

页数：7

共 8 条

[1] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].