Possible role of Cbr, an algal early-light-induced protein, in nonphotochemical quenching of chlorophyll fluorescence

被引:17
作者
Braun, P [1 ]
Banet, G [1 ]
Tal, T [1 ]
Malkin, S [1 ]
Zamir, A [1 ]
机构
[1] WEIZMANN INST SCI,DEPT BIOCHEM,IL-76100 REHOVOT,ISRAEL
关键词
D O I
10.1104/pp.110.4.1405
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The unicellular green alga Dunaliella bardawil exhibits typical responses to excessive light when starved for sulfate under normal light (60 mu E m(-2) s(-1)) but not under low light (14 mu E m(-2) s(-1)). Algae were analyzed during several days of sulfate starvation for nonphotochemical quenching of chlorophyll fluorescence in the absence or presence of the uncouplers SF-6847 (SF) or carbonyl cyanide p-trifluoromethoxyphenyl hydrazone. Parallel analyses followed two light-stress responses: (a) violaxanthin conversion to zeaxanthin and (b) accumulation of Cbr, a protein analogous to plant early-light-induced proteins and implicated in zeaxanthin binding. In cells starved under normal light SF inhibited nonphotochemical quenching during the first 24 h, but not from 40 h onward. In cells starved under low light SF inhibited nonphotochemical quenching throughout the starvation period. Under normal light accumulation of zeaxanthin was nearly maximal by 24 h, but Cbr was fully induced only by 40 h. Under low light zeaxanthin accumulated slowly but no Cbr was evident. These results suggest that during exposure to excessive light, the initial pH gradient-dependent, Cbr-independent mode of nonphotochemical quenching is modified to become less dependent on pH gradient and requires Cbr.
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页码:1405 / 1411
页数:7
相关论文
共 24 条
[1]  
ADAMSKA I, 1992, J BIOL CHEM, V267, P24732
[2]   SHORT-TERM ADAPTATION OF PLANTS TO CHANGING LIGHT INTENSITIES AND ITS RELATION TO PHOTOSYSTEM-II PHOTOCHEMISTRY AND FLUORESCENCE EMISSION [J].
DAU, H .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1994, 26 (01) :3-27
[3]   CAROTENOIDS AND PHOTOPROTECTION IN PLANTS - A ROLE FOR THE XANTHOPHYLL ZEAXANTHIN [J].
DEMMIGADAMS, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1020 (01) :1-24
[4]   PHOTOPROTECTION AND OTHER RESPONSES OF PLANTS TO HIGH LIGHT STRESS [J].
DEMMIGADAMS, B ;
ADAMS, WW .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1992, 43 :599-626
[5]  
GREEN B, 1991, TRENDS BIOCHEM SCI, V161, P181
[6]   THE EARLY LIGHT-INDUCIBLE PROTEINS OF BARLEY - CHARACTERIZATION OF 2 FAMILIES OF 2-H-SPECIFIC NUCLEAR-CODED CHLOROPLAST PROTEINS [J].
GRIMM, B ;
KLOPPSTECH, K .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 167 (03) :493-499
[7]   REGULATION OF LIGHT-HARVESTING IN GREEN PLANTS - INDICATION BY NONPHOTOCHEMICAL QUENCHING OF CHLOROPHYLL FLUORESCENCE [J].
HORTON, P ;
RUBAN, AV ;
WALTERS, RG .
PLANT PHYSIOLOGY, 1994, 106 (02) :415-420
[8]   REGULATION OF PHOTOSYSTEM-II [J].
HORTON, P ;
RUBAN, AV .
PHOTOSYNTHESIS RESEARCH, 1992, 34 (03) :375-385
[9]   DELTA-PH-DEPENDENT CHLOROPHYLL FLUORESCENCE QUENCHING INDICATING A MECHANISM OF PROTECTION AGAINST PHOTOINHIBITION OF CHLOROPLASTS [J].
KRAUSE, GH ;
BEHREND, U .
FEBS LETTERS, 1986, 200 (02) :298-302
[10]  
LERS A, 1991, J BIOL CHEM, V266, P13698