Simple method for determination of hydrochlorothiazide in human urine by high performance liquid chromatography utilizing narrowbore chromatography

被引：49

作者：

Farthing, D ^{[1
]}

Fakhry, I ^{[1
]}

Ripley, EBD ^{[1
]}

Sica, D ^{[1
]}

机构：

[1] Virginia Commonwealth Univ, Med Coll Virginia, Div Clin Pharmacol & Hypertens, Richmond, VA 23298 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1998年 / 17卷 / 08期

关键词：

hydrochlorothiazide; urine; narrowbore HPLC;

D O I：

10.1016/S0731-7085(98)00021-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple high performance liquid chromatographic (HPLC) method utilizing narrowbore chromatography was developed for the determination of hydrochlorothiazide in human urine. A mobile phase of 0.1% aqueous acetic acid-acetonitrile (93:7, v/v) pH 3 was used with a C18 analytical column and ultraviolet detection (UV). The method demonstrated linearity from 2 to 50 mu g ml(-1) using 50 mu l of urine with a detection limit of 1 mu g ml(-1). The method was utilized in a study evaluating if racial differences are present in the pharmacokinetic and pharmacodynamic effects of hydrochlorothiazide. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：1455 / 1459

页数：5

共 10 条

[1] HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CHLOROTHIAZIDE AND HYDROCHLOROTHIAZIDE IN PLASMA AND URINE - PRELIMINARY-RESULTS OF CLINICAL-STUDIES [J].