Recent advances in site-directed spin labeling of proteins

被引：481

作者：

Hubbell, WL ^{[1
]}

Gross, A

Langen, R

Lietzow, MA

机构：

[1] Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1998年 / 8卷 / 05期

关键词：

D O I：

10.1016/S0959-440X(98)80158-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Site-directed spin labeling of proteins is experiencing a phase of rapid technical evolution, application and evaluation, New strategies have been introduced for determining membrane protein topography, electrostatic potentials, the orientation of proteins at membrane surfaces and inter-residue distances, New applications include studies of beta strands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of membrane and soluble proteins.

引用

页码：649 / 656

页数：8

共 34 条

[1] Estimating the electrostatic potential at the acetylcholine receptor agonist site using power saturation EPR
Addona, GH
Andrews, SH
Cafiso, DS
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1329 (01): : 74 - 84
[2] Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: A site-directed spin-labeling study
Altenbach, C
Yang, K
Farrens, DL
Farahbakhsh, ZT
Khorana, HG
Hubbell, WL
[J]. BIOCHEMISTRY, 1996, 35 (38) : 12470 - 12478
[3] A large and distinct rotation of the myosin light chain domain occurs upon muscle contraction
Baker, JE
Brust-Mascher, I
Ramachandran, S
LaConte, LEW
Thomas, DD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (06) : 2944 - 2949
[4] Structure and function of the conserved domain in alpha A-crystallin. Site-directed spin labeling identifies a beta-strand located near a subunit interface
Berengian, AR
Bova, MP
Mchaourab, HS
[J]. BIOCHEMISTRY, 1997, 36 (33) : 9951 - 9957
[5] Structure and function in rhodopsin: Topology of the C-terminal polypeptide chain in relation to the cytoplasmic loops
Cai, KW
Langen, R
Hubbell, WL
Khorana, HG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (26) : 14267 - 14272
[6] The structure of the potassium channel:: Molecular basis of K+ conduction and selectivity
Doyle, DA
Cabral, JM
Pfuetzner, RA
Kuo, AL
Gulbis, JM
Cohen, SL
Chait, BT
MacKinnon, R
[J]. SCIENCE, 1998, 280 (5360) : 69 - 77
[7] Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin
Farrens, DL
Altenbach, C
Yang, K
Hubbell, WL
Khorana, HG
[J]. SCIENCE, 1996, 274 (5288) : 768 - 770
[8] Two modes of ligand binding in maltose-binding protein of Escherichia coli - Electron paramagnetic resonance study of ligand-induced global conformational changes by site-directed spin labeling
Hall, JA
Thorgeirsson, TE
Liu, J
Shin, YK
Nikaido, H
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (28) : 17610 - 17614
[9] Arginine 302 (helix IX) in the lactose permease of Escherichia coli is in close proximity to glutamate 269 (helix VIII) as well as glutamate 325 (helix X)
He, MM
Voss, J
Hubbell, WL
Kaback, HR
[J]. BIOCHEMISTRY, 1997, 36 (44) : 13682 - 13687
[10] Watching proteins move using site-directed spin labeling
Hubbell, WL
Mchaourab, HS
Altenbach, C
Lietzow, MA
[J]. STRUCTURE, 1996, 4 (07) : 779 - 783

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