Serotonin depresses excitatory synaptic transmission and depolarization-evoked Ca2+ influx in rat basolateral amygdala via 5-HT1A receptors

被引:96
作者
Cheng, LL [1 ]
Wang, SJ [1 ]
Gean, PW [1 ]
机构
[1] Natl Cheng Kung Univ, Coll Med, Dept Pharmacol, Tainan 701, Taiwan
关键词
amygdala; excitatory postsynaptic potential; serotonin; synaptic transmission;
D O I
10.1046/j.1460-9568.1998.00229.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The actions of serotonin on rat basolateral amygdala neurons were studied with conventional intracellular recording techniques and fura-e fluorimetric recordings. Bath application of 5-hydroxytryptamine (5-HT or serotonin) reversibly suppressed the excitatory postsynaptic potential in a concentration-dependent manner without affecting the resting membrane potential and neuronal input resistance. Extracellular Ba2+ or pertussis toxin pretreatment did not affect the depressing effect of 5-HT suggesting that it is not mediated through activation of G(i/o), protein-coupled K+ conductance, The sensitivity of postsynaptic neurons to glutamate receptor agonist was unaltered by the 5-HT pretreatment, In addition, the magnitude of paired-pulse facilitation was increased in the presence of 5-HT indicating a presynaptic mode of action. The effect of 5-HT was mimicked by the selective 5-HT1A agonist 8-hydroxy-dipropylaminotetralin (8-OH-DPAT) and was blocked by the selective 5-HT1A antagonist 1-(2-methoxyphenyl)-4[4-(2-phthalimido)butyl]piperazine oxadiazol-3-yl]methyl]phenyl]-methanesulphonamide. In contrast, the selective 5-HT2 receptor antagonist ketanserin failed to affect the action of 5-MT. The effects of 5-HT and 8-OH-DPAT on the high K+-induced increase in [Ca2+](i) were studied in acutely dissociated basolateral amygdala neurons. High K+-induced increase in [Ca2+](i) was blocked by Ca2+-free solution and Cd2+ suggesting that Ca2+ entry responsible for the depolarizaton-evoked increase in [Ca2+](i) occurred through voltage-dependent Ca2+ channels. Application of 5-HT and 8-OH-DPAT reduced the K+-induced Ca2+ influx in a concentration-dependent manner. The effect of 5-HT was completely abolished in slices pretreated with Rp-cyclic adenosine 3',5'-monophosphothioate (Rp-cAMP), a regulatory site antagonist of protein kinase A, suggesting that 5-HT may act through a cAMP-dependent mechanism. Taken together, these results suggest that functional 5-HT1A receptors are present in the excitatory terminals and mediate the 5-HT inhibition of synaptic transmission in the amygdala.
引用
收藏
页码:2163 / 2172
页数:10
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