DNA determination mediates innate immunity to retroviral infection

被引：1119

作者：

Harris, RS

Bishop, KN

Sheehy, AM

Craig, HM

Petersen-Mahrt, SK

Watt, IN

Neuberger, MS

Malim, MH

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Kings Coll London, Guys Kings & St Thomas Sch Med, Dept Infect Dis, London SE1 9RT, England

来源：

CELL | 2003年 / 113卷 / 06期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0092-8674(03)00423-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CEM15/APOBEC3G is a cellular protein required for resistance to infection by virion infectivity factor (Vif)-deficient human immunodeficiency virus (HIV). Here, using a murine leukemia virus (MLV)-based system, we provide evidence that CEM15/APOBEC3G is a DNA deaminase that is incorporated into virions during viral production and subsequently triggers massive deamination of deoxycytidine to deoxyuridine within the retroviral minus (first)-strand cDNA, thus providing a probable trigger for viral destruction. Furthermore, HIV Vif can protect MLV from this CEM15/APOBEC3G-dependent restriction. These findings imply that targeted DNA deamination is a major strategy of innate immunity to retroviruses and likely also contributes to the sequence variation observed in many viruses (including HIV).

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收藏

页码：803 / 809

页数：7

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