Design and evaluation of an in-house HIV-1 (group M and O), SIVmnd and SIVcpz antigen capture assay

被引:43
作者
Beirnaert, E
Willems, B
Peeters, M
Bouckaert, A
Heyndrickx, L
Zhong, P
Vereecken, K
Coppens, S
Davis, D
Ndumbe, P
Janssens, W
van der Groen, G
机构
[1] Inst Trop Med, Dept Microbiol, B-2000 Antwerp, Belgium
[2] ORSTOM, SIDA, Retrovirus Lab, Montpellier, France
[3] Shanghai Inst Biol Prod, Shanghai, Peoples R China
[4] Univ Yaounde, Ctr Study & Control Communicable Dis, Yaounde, Cameroon
关键词
HIV; SIV; antigen detection;
D O I
10.1016/S0166-0934(98)00044-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An enzyme-linked immuno-sorbent assay (ELISA) for the detection of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIVcpz/SIVmnd) antigens was designed using immunoreagents from naturally infected individuals, and compared to the commercially available Vironostika HIV-1 Antigen Microelisa System (Organon Teknika). The in-house assay proved to be specific for HIV-1 isolates belonging to group M (A-H) and group O and for SIVcpz and SIVmnd isolates, but was less sensitive than the Vironostika HIV-1 Antigen Microelisa System, except for SIVmnd. For the strains belonging to HIV-2, SIVmac and SIVagm, the in-house assay could not detect antigen to an appreciable degree. This study shows that a considerably less expensive but sufficiently accurate HIV-1 antigen capture assay can be developed to monitor HIV-1 (group M and O), SIVcpz and SIVmnd, antigen in the supernatants of virus cultures. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:65 / 70
页数:6
相关论文
共 19 条
[1]   GENETIC-RELATIONSHIPS DETERMINED BY A DNA HETERODUPLEX MOBILITY ASSAY - ANALYSIS OF HIV-1 ENV GENES [J].
DELWART, EL ;
SHPAER, EG ;
LOUWAGIE, J ;
MCCUTCHAN, FE ;
GREZ, M ;
RUBSAMENWAIGMANN, H ;
MULLINS, JI .
SCIENCE, 1993, 262 (5137) :1257-1261
[2]  
DEWOLF F, 1988, J INFECT DIS, V158, P615, DOI 10.1093/infdis/158.3.615
[3]   Longstanding presence in Belgians of multiple non-B HIV-1 subtypes [J].
Fransen, K ;
Buve, A ;
Nkengasong, JN ;
Laga, M ;
vanderGroen, G .
LANCET, 1996, 347 (9012) :1403-1403
[4]   PATHOGENESIS OF HIV AND ITS IMPLICATIONS FOR SERODIAGNOSIS AND MONITORING OF ANTIVIRAL THERAPY [J].
GOUDSMIT, J ;
LANGE, JMA ;
KRONE, WJA ;
TEUNISSEN, MBM ;
EPSTEIN, LG ;
DANNER, SA ;
VANDENBERG, H ;
BREEDERVELD, C ;
SMIT, L ;
BAKKER, M ;
DEWOLF, F ;
COUTINHO, RA ;
VANDERNOORDAA, J .
JOURNAL OF VIROLOGICAL METHODS, 1987, 17 (1-2) :19-34
[5]  
GURTLER LG, 1994, J VIROL, V68, P1581
[6]   USE OF VIROLOGICAL ASSAYS FOR DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS IN CLINICAL-TRIALS - RECOMMENDATIONS OF THE AIDS CLINICAL-TRIALS GROUP VIROLOGY COMMITTEE [J].
HAMMER, S ;
CRUMPACKER, C ;
DAQUILA, R ;
JACKSON, B ;
LATHEY, J ;
LIVNAT, D ;
REICHELDERFER, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (10) :2557-2564
[7]   CONVERGENT AND DIVERGENT SEQUENCE EVOLUTION IN THE SURFACE ENVELOPE GLYCOPROTEIN OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 WITHIN A SINGLE INFECTED PATIENT [J].
HOLMES, EC ;
ZHANG, LQ ;
SIMMONDS, P ;
LUDLAM, CA ;
BROWN, AJL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4835-4839
[8]   The puzzle of HIV-1 subtypes in Africa [J].
Janssens, W ;
Buve, A ;
Nkengasong, JN .
AIDS, 1997, 11 (06) :705-712
[9]  
JANSSENS W, 1900, UNPUB INTER INTRAPAT
[10]   PHYLOGENETIC ANALYSIS OF GAG GENES FROM 70 INTERNATIONAL HIV-1 ISOLATES PROVIDES EVIDENCE FOR MULTIPLE GENOTYPES [J].
LOUWAGIE, J ;
MCCUTCHAN, FE ;
PEETERS, M ;
BRENNAN, TP ;
SANDERSBUELL, E ;
EDDY, GA ;
VANDERGROEN, G ;
FRANSEN, K ;
GERSHYDAMET, GM ;
DELEYS, R ;
BURKE, DS .
AIDS, 1993, 7 (06) :769-780