Combinatorial protein design by in vitro recombination

被引：36

作者：

Giver, L ^{[1
]}

Arnold, FH ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn 210 41, Pasadena, CA 91125 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 1998年 / 2卷 / 03期

关键词：

D O I：

10.1016/S1367-5931(98)80006-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA recombination is a powerful engine for the creation of new phenotypes. Recently, methods for in vitro DNA recombination (DNA shuffling) have been developed and applied to the evolution of novel molecules in the laboratory. An exciting new development is the shuffling of homologous genes to create diversity for directed evolution.

引用

页码：335 / 338

页数：4

共 26 条

[1] AN ALGORITHM FOR PROTEIN ENGINEERING - SIMULATIONS OF RECURSIVE ENSEMBLE MUTAGENESIS [J].

ARKIN, AP ;

YOUVAN, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) :7811-7815

[2] Design by directed evolution [J].

Arnold, FH .

ACCOUNTS OF CHEMICAL RESEARCH, 1998, 31 (03) :125-131

[3] Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy [J].

Black, ME ;

Newcomb, TG ;

Wilson, HMP ;

Loeb, LA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (08) :3525-3529

[4] Improved green fluorescent protein by molecular evolution using DNA shuffling [J].

Crameri, A ;

Whitehorn, EA ;

Tate, E ;

Stemmer, WPC .

NATURE BIOTECHNOLOGY, 1996, 14 (03) :315-319

[5] Construction and evolution of antibody-phage libraries by DNA shuffling [J].

Crameri, A ;

Cwirla, S ;

Stemmer, WPC .

NATURE MEDICINE, 1996, 2 (01) :100-102

[6] Molecular evolution of an arsenate detoxification pathway DNA shuffling [J].

Crameri, A ;

Dawes, G ;

Rodriguez, E ;

Silver, S ;

Stemmer, WPC .

NATURE BIOTECHNOLOGY, 1997, 15 (05) :436-438

[7] DNA shuffling of a family of genes from diverse species accelerates directed evolution [J].

Crameri, A ;

Raillard, SA ;

Bermudez, E ;

Stemmer, WPC .

NATURE, 1998, 391 (6664) :288-291

[8] SEARCHING SEQUENCE SPACE TO ENGINEER PROTEINS - EXPONENTIAL ENSEMBLE MUTAGENESIS [J].

DELAGRAVE, S ;

YOUVAN, DC .

BIO-TECHNOLOGY, 1993, 11 (13) :1548-1552

[9]

Hurst Laurence D., 1996, Trends in Ecology and Evolution, V11, P46, DOI 10.1016/0169-5347(96)81041-X

[10] Smoothness within ruggedness: The role of neutrality in adaptation [J].

Huynen, MA ;

Stadler, PF ;

Fontana, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (01) :397-401

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