Binding of the N-terminal 63 kDa portion of connectin/titin to alpha-actinin as revealed by the yeast two-hybrid system

被引：72

作者：

Ohtsuka, H ^{[1
]}

Yajima, H ^{[1
]}

Maruyama, K ^{[1
]}

Kimura, S ^{[1
]}

机构：

[1] CHIBA UNIV,FAC SCI,DEPT BIOL,CHIBA 263,JAPAN

来源：

FEBS LETTERS | 1997年 / 401卷 / 01期

关键词：

connectin; titin; alpha-actinin; muscle structure; Z-line; two-hybrid system; enzyme-linked immunosorbent assay;

D O I：

10.1016/S0014-5793(96)01432-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z-line in vertebrate striated muscle sarcomeres, To search for the Z-line proteins to which connectin binds, the yeast two-hybrid system was applied using cDNA coding the N-terminal 63 kDa fragment of connectin, Two clones coding the C-terminal half region of alpha-actinin (amino acids, 343-897 and 446-897) were obtained. Enzyme-linked immunosorbent assay clearly demonstrated the interactions of alpha-actinin and the N-terminal 63 kDa fragment of connectin in vitro, Thus it is concluded that the N-terminal 63 kDa portion of connectin binds to alpha-actinin in the Z-line of myofibrillar sarcomeres.

引用

页码：65 / 67

页数：3

共 17 条

[1]

PRIMARY STRUCTURE OF CHICKEN SKELETAL-MUSCLE AND FIBROBLAST ALPHA-ACTININS DEDUCED FROM CDNA SEQUENCES [J].