Modulation of adipocytokines response and weight loss secondary to a hypocaloric diet in obese patients by-55CT polymorphism of UCP3 gene

被引:18
作者
de Luis, D. A.
Aller, R.
Izaola, O.
Sagrado, M. G.
Conde, R.
机构
[1] Univ Valladolid, Sch Med, Inst Endocrinol & Nutr, Hosp Rio Hortega, Valladolid 47130, Spain
[2] Univ Valladolid, Hosp Rio Hortega, Unit Invest, Valladolid 47130, Spain
关键词
55CT polymorphism of UCP3 gene; diet; obesity;
D O I
10.1055/s-2008-1046796
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Decreased expression or function of UCP3 (uncoupling protein 3) could reduce energy expenditure and increase the storage of energy as fat. Some studies have pointed to a role of UCP3 in the regulation of whole body energy homeostasis, diet induced obesity, and regulation of lipids as metabolic substrates. The C/C genotype of a polymorphism in the UCP3 promoter (-55C -> T) is associated with an increased expression of UCP3 mRNA in muscle. The aim of our study was to investigate the influence of -55CT polymorphism of UCP3 gene on adipocytokines response and weight loss secondary to a hypocaloric diet in obese patients. A population of 107 obese (body mass index >30) nondiabetic outpatients was analyzed in a prospective way. Before and after three months of a hypocaloric diet, an indirect calorimetry, tetrapolar electrical bioimpedance, blood pressure, a serial assessment of nutritional intake with 3-day written food records, and biochemical analysis were performed. The lifestyle modification program consisted of a hypocaloric diet (1520 kcal, 52% of carbohydrates, 25% of lipids and 23% of proteins). The exercise program consisted of aerobic exercise for at least 3 times per week (60 minutes each). The mean age was 49.5 +/- 34.5 years and the mean BMI 34.5 +/- 4.8, with 27 males (25.3%) and 80 females (74.7%). Ninety patients (25 males/65 females) (83.6%) had the genotype 55CC (wild group) and 17 patients (2 male/15 females) (16.4%) 55CT (mutant group). The percentage of responders (weight loss) was similar in both groups (wild group: 84.7% vs. mutant group: 81.8%). BMI, weight, fat mass, systolic blood pressure, LDL cholesterol, waist circumference, and waist-to-hip ratio decreased in the wild group and RMR and VO2 were increased. In the mutant group, BMI and weight decreased. Leptin and IL-6 levels have a significant decrease in the wild group (9.6%: p < 0.05) and (30.5%: p < 0.05), respectively. Patients with -55CC genotype have a significant decrease in leptin, interleukin 6, BMI, weight, fat mass, systolic blood pressure, LDL cholesterol, waist circumference, waist-to-hip ratio weight, fat mass, and systolic blood pressure.
引用
收藏
页码:214 / 218
页数:5
相关论文
共 31 条
[1]   Association of UCP3 gene -55C&gt;T polymorphism and obesity in a Spanish population -: A case-control study [J].
Alonso, A ;
Martí, A ;
Corbalán, MS ;
Martínez-González, MA ;
Forga, L ;
Martínez, JA .
ANNALS OF NUTRITION AND METABOLISM, 2005, 49 (03) :183-188
[2]  
Aranceta J, 1998, MED CLIN-BARCELONA, V111, P441
[3]   Elevated levels of interleukin 6 are reduced in serum and subcutaneous adipose tissue of obese women after weight loss [J].
Bastard, JP ;
Jardel, C ;
Bruckert, E ;
Blondy, P ;
Capeau, J ;
Laville, M ;
Vidal, H ;
Hainque, B .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2000, 85 (09) :3338-3342
[4]   Linkage between markers in the vicinity of the uncoupling protein 2 gene and resting metabolic rate in humans [J].
Bouchard, C ;
Perusse, L ;
Chagnon, YC ;
Warden, C ;
Ricquier, D .
HUMAN MOLECULAR GENETICS, 1997, 6 (11) :1887-1889
[5]   Evidence that single nucleotide polymorphism in the uncoupling protein 3 (UCP3) gene influences fat distribution in women of European and Asian origin [J].
Cassell, PG ;
Saker, PJ ;
Huxtable, SJ ;
Kousta, E ;
Jackson, AE ;
Hattersley, AT ;
Frayling, TM ;
Walker, M ;
Kopelman, PG ;
Ramachandran, A ;
Snehelatha, C ;
Hitman, GA ;
McCarthy, MI .
DIABETOLOGIA, 2000, 43 (12) :1558-1564
[6]   Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean [J].
Clapham, JC ;
Arch, JRS ;
Chapman, H ;
Haynes, A ;
Lister, C ;
Moore, GBT ;
Piercy, V ;
Carter, SA ;
Lehner, I ;
Smith, SA ;
Beeley, LJ ;
Godden, RJ ;
Herrity, N ;
Skehel, M ;
Changani, KK ;
Hockings, PD ;
Reid, DG ;
Squires, SM ;
Hatcher, J ;
Trail, B ;
Latcham, J ;
Rastan, S ;
Harper, AJ ;
Cadenas, S ;
Buckingham, JA ;
Brand, MD ;
Abuin, A .
NATURE, 2000, 406 (6794) :415-418
[7]  
COMMINS SP, 2005, J PHYSL ENDOCRINOL M, V280, P372
[8]   Regulation of skeletal muscle UCP-2 and UCP-3 gene expression by exercise and denervation [J].
Cortright, RN ;
Zheng, DH ;
Jones, JP ;
Fluckey, JD ;
DiCarlo, SE ;
Grujic, D ;
Lowell, BB ;
Dohm, GL .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1999, 276 (01) :E217-E221
[9]  
DAIGUARD LT, 2001, J CLIN ENDOCR METAB, V86, P1398
[10]   Uncoupling protein 2 region on chromosome 11q13 is not linked to markers of obesity in familial type 2 diabetes [J].
Elbein, SC ;
Leppert, M ;
Hasstedt, S .
DIABETES, 1997, 46 (12) :2105-2107