Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis

被引：37

作者：

Edwards, KJ

Metherell, LA

Yates, M

Saunders, NA

机构：

[1] Cent Publ Hlth Lab, Div Virus Reference, Mol Biol Unit, London NW9 5HT, England

[2] Dulwich Hosp, Dulwich Publ Hlth Lab, PHLS Mycobacterium Reference Unit, London SE22 8QF, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 09期

关键词：

D O I：

10.1128/JCM.39.9.3350-3352.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.

引用

收藏

页码：3350 / 3352

页数：3

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