PCR detection of Listeria monocytogenes in 'gravad' rainbow trout

被引：15

作者：

Ericsson, H

Stalhandske, P

机构：

[1] Department of Food Hygiene, Facility of Veterinary Medicine, Swed. Univ. of Agric. Sciences

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1997年 / 35卷 / 03期

关键词：

L-monocytogenes; gravad rainbow trout; PCR;

D O I：

10.1016/S0168-1605(97)01246-4

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

'Gravad' rainbow trout artificially contaminated with Listeria monocytogenes was analyzed by use of a 4 h enrichment period followed by extraction of DNA and PCR amplification. This procedure made it possible to detect 10-100 cfu L. monocytogenes per gram 'gravad' rainbow trout, within 12 h. After a prolonged enrichment period of 24 h, numbers as low as 1-10 cfu L. monocytogenes per gram could be detected. The method described may be a useful tool for screening samples of 'gravad' rainbow trout for the presence of L. monocytogenes, since it is sensitive, rapid and simple. (C) 1997 Elsevier Science B.V.

引用

页码：281 / 285

页数：5

共 16 条

[1]

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[2]

FISHER PA, 1979, J BIOL CHEM, V254, P1040

[3] DETECTION AND IDENTIFICATION OF LISTERIA-MONOCYTOGENES IN COOKED SAUSAGE PRODUCTS AND IN MILK BY INVITRO AMPLIFICATION OF HEMOLYSIN GENE FRAGMENTS [J].