Mechanism of hyperbaric oxygen preconditioning in neonatal hypoxia-ischemia rat model

被引：32

作者：

Li, Zhang

Liu, Wenwu ^{[1
]}

Kang, Zhimin ^{[1
]}

Lv, Shijun

Han, Cuihong

Yun, Liu ^{[1
]}

Sun, Xuejun ^{[1
]}

Zhang, John H. ^{[2
]}

机构：

[1] Second Mil Med Univ, Fac Naval Med, Dept Diving Med, Shanghai 200433, Peoples R China

[2] Loma Linda Univ, Dept Neurosurg, Loma Linda, CA 92350 USA

来源：

BRAIN RESEARCH | 2008年 / 1196卷

关键词：

hyperbaric oxygen preconditioning; hypoxic ischemia; apoptosis;

D O I：

10.1016/j.brainres.2007.12.039

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Hypoxic ischemic (HI) injury in neonates damages brain tissues. We examined the mechanism of hyperbaric oxygen preconditioning (HBO-PC) in neonatal HI rat model. Seven-day-old rat pups were subjected to left common carotid artery ligation and hypoxia (8% oxygen at 37 degrees C) for 90 min. HBO (100% O-2, 2.5 atmospheres absolute for 2.5 h) were administered by placing pups in a chamber 24 h before HI insult. Brain injury was assessed by the survival rate, 2,3,5-triphenyltetrazolium chloride (TTC), Nissl, TUNEL straining and caspase-3,caspase-9 activities after HI. In HBO preconditioned animals, survival rate was increased, infarct ratio was decreased, and the positive stained TUNEL cells were reduced, accompanied by the suppression of caspase-3 and -9 activities. These results indicate that a single HBO-PC appears to provide brain protection against HI insult via inhibition of neuronal apoptosis pathways. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：151 / 156

页数：6

共 33 条

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