Photoreaction cycle of photoactive yellow protein from Ectothiorhodospira halophila studied by low-temperature spectroscopy

被引:122
作者
Imamoto, Y [1 ]
Kataoka, M [1 ]
Tokunaga, F [1 ]
机构
[1] OSAKA UNIV,GRAD SCH SCI,DEPT EARTH & SPACE SCI,TOYONAKA,OSAKA 560,JAPAN
关键词
D O I
10.1021/bi961342d
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The photocycle of photoactive yellow protein (PYP) from Ectothiorhodospira halophila was studied by low-temperature spectroscopy. Irradiation of PYP at -190 degrees C produced a photo-steady-state mixture composed of bathochromic and hypsochromic photoproducts (PYPB and PYPH). Upon warming, PYPH was thermally converted to a slightly blue-shifted intermediate (PYPHL) above -150 degrees C and then to a red-shifted one (PYPL) above -80 degrees C. PYPB was thermally converted to the blue-shifted intermediate (PYPBL) above -180 degrees C and then to PYPL above -90 degrees C. PYPL thermally reverted to PYP above -50 degrees C, completing the photocycle. The spectral properties of PYPL formed at low temperature suggest that it corresponds to the red-shifted photoproduct detected in the nano- to microsecond time scale at room temperature (A(465)) The absolute absorption spectra of PYPH, PYPB, and PYPL were estimated, and their absorption maxima were determined to be 442 and 489 nm at -190 degrees C and 456 nm at -80 degrees C, respectively. Although a near-UV intermediate (A(355)) is observed in the recovery process of PYP from A(465) at room temperature, it was not detected at low temperatures, probably due to the effects of temperature and the presence of glycerol. A scheme of the photocycle of PYP is presented.
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页码:14047 / 14053
页数:7
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