Employment of 16S rDNA gene sequencing techniques to identify phenotypically difficult-to-identify culturable eubacteria from foods and waters

A molecular f(DNA) method based on 16S rDNA polymerase chain reaction (PCR) was developed by employing universal oligonucleotide primers followed by direct automated sequencing of the PCR amplicon. The employment of the methodologies allowed for the reliable identification of collection of eubacteria isolated from several food and water sources. Highly variable portions of the 16S rRNA sequence provided unique signatures to any bacterium and useful information about relationships between them. Employment of partial 16S rDNA PCR and sequencing provided a valuable and reliable method of identification of environmental bacteria associated with food and water.