Electro-ultrafiltration of industrial enzyme solutions

被引：48

作者：

Enevoldsen, A. D.

Hansen, E. B.

Jonsson, G. ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Chem Engn, DK-2800 Lyngby, Denmark

[2] Novozymes AS, Recovery Dev, DK-2880 Bagsvaerd, Denmark

来源：

JOURNAL OF MEMBRANE SCIENCE | 2007年 / 299卷 / 1-2期

关键词：

crossflow filtration; enzyme; electrophoretic mobility; electrophoresis; electrofiltration; electric field; membrane fouling;

D O I：

10.1016/j.memsci.2007.04.021

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

To reduce the problems with fouling and concentration polarization during crossflow ultrafiltration of industrial enzyme solutions an electric field is applied across the membrane. The filtration performance during electro-ultrafiltration (EUF) has been tested with several enzymes. Results show that EUF is an effective method to filter high concentrated solutions at low crossfiow. The flux improved 3-7 times for enzymes with a significant surface charge at an electric field strength of 1600V/m compared to conventional UF. The greatest improvement is observed at high concentration. Not all enzymes can be filtered with EUF, mainly due to a low surface charge and impurities in the feed solution. Using a pulsed electric field did not improve the flux compared to a constant field. Gel electrophoresis experiments of the enzymes appear to be a useful method for estimating the influence of the electric field. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：28 / 37

页数：10

共 35 条

[1]

Bargeman G, 2000, LAIT, V80, P175, DOI 10.1051/lait:2000117

[2] Electro-membrane filtration for the selective isolation of bioactive peptides from an αs2-casein hydrolysate [J].

Bargeman, G ;

Houwing, J ;

Recio, I ;

Koops, GH ;

van der Horst, C .

BIOTECHNOLOGY AND BIOENGINEERING, 2002, 80 (06) :599-609

[3] THE BEHAVIOR OF SUSPENSIONS AND MACROMOLECULAR SOLUTIONS IN CROSS-FLOW MICROFILTRATION [J].

BELFORT, G ;

DAVIS, RH ;

ZYDNEY, AL .

JOURNAL OF MEMBRANE SCIENCE, 1994, 96 (1-2) :1-58

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Flux enhancement in cross-flow membrane filtration by flow reversal: A case study on ultrafiltration of BSA [J].

Hargrove, SC ;

Parthasarathy, H ;

Ilias, S .

SEPARATION SCIENCE AND TECHNOLOGY, 2003, 38 (12-13) :3133-3144

[6] SOLID-LIQUID SEPARATION PROCESS BASED ON CROSS FLOW AND ELECTROFILTRATION [J].

HENRY, JD ;

LAWLER, LF ;

KUO, CHA .

AICHE JOURNAL, 1977, 23 (06) :851-859

[7] The effect of protein-protein and protein-membrane interactions on membrane fouling in ultrafiltration [J].

Huisman, IH ;

Prádanos, P ;

Hernández, A .

JOURNAL OF MEMBRANE SCIENCE, 2000, 179 (1-2) :79-90

[8] Electrically enhanced crossflow membrane filtration of oily waste water using the membrane as a cathode [J].

Huotari, HM ;

Huisman, IH ;

Trägårdh, G .

JOURNAL OF MEMBRANE SCIENCE, 1999, 156 (01) :49-60

[9]

HUOTARI HM, 1999, T ICHEME A, V77, P461

[10]

Kelly ST, 1997, BIOTECHNOL BIOENG, V55, P91, DOI [10.1002/(SICI)1097-0290(19970705)55:1&lt

← 1 2 3 4 →