DNA methylation, ageing and ribosomal genes activity

被引：10

作者：

de Carvalho, CV ^{[1
]}

Payao, SLM ^{[1
]}

Smith, MD ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Escola Paulista Med, UNIFESP, EPM,Dept Morfol,Disciplina Genet, BR-04023900 Sao Paulo, Brazil

来源：

BIOGERONTOLOGY | 2000年 / 1卷 / 04期

关键词：

ageing; ribosomal genes; Alzheimer's disease; DNA methylation; nucleolus organizer region;

D O I：

10.1023/A:1026542618182

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

DNA methylation is an epigenetic mechanism controlling of gene expression. We studied the effect of a demethylation agent 5-azacytidine, on ribosomal gene activity observed by Nucleolus Staining Giemsa in the chromosomes of eight Alzheimer's disease patients, eight elderly healthy individuals and eight young healthy controls. Metaphase cells were obtained from lymphocyte cultures. All the acrocentric chromosome pairs were analyzed using G banding followed by Nucleolus Staining Giemsa banding in the same cell. The Alzheimer's disease patients and elderly healthy control did not show a variation in the transcriptional activity of ribosomal genes with or without 5-azacytidine treatment, whereas the young control group showed an increase in this activity with the treatment.

引用

页码：357 / 361

页数：5

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