Improvement of intracellular β-galactosidase production in fed-batch culture of Kluyveromyces fragilis

被引：33

作者：

Nor, ZM ^{[1
]}

Tamer, MI ^{[1
]}

Mehrvar, M ^{[1
]}

Scharer, JM ^{[1
]}

Moo-Young, M ^{[1
]}

Jervis, EJ ^{[1
]}

机构：

[1] Univ Waterloo, Dept Chem Engn, Waterloo, ON N2L 3G1, Canada

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 11期

关键词：

fed-batch control; Kluyveromyces fragilis; lactase; on-line specific growth rate estimation;

D O I：

10.1023/A:1010526831985

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Four fed-batch control strategies were evaluated to improve the specific lactase activity of Kluyveromyces fragilis. Control strategies tested included DO-stat control, exponential feeding, exponential feeding with manual feedback control and corrected feed-forward control. Each was implemented with standard sensors (i.e., temperature, dissolved oxygen and pH sensors) commonly installed in fermenters. The highest specific activity was obtained using the corrected feed-forward control strategy, a strategy incorporating a novel method for on-line estimation of specific growth rate. The control strategy was able to operate effectively to a final cell density of 69 g dry wt l(-1) with a specific lactase activity of 2 U mg(-1) cell dry wt.

引用

页码：845 / 849

页数：5

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