The Listeria monocytogenes prf AP2 promoter by sigma B in a growth phase dependent is regulated manner

被引：54

作者：

Schwab, U ^{[1
]}

Bowen, B ^{[1
]}

Nadon, C ^{[1
]}

Wiedmann, M ^{[1
]}

Boor, KJ ^{[1
]}

机构：

[1] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 245卷 / 02期

关键词：

Listeria monocytogenes; prf A; sigma B; gas Reporter gene;

D O I：

10.1016/j.femsle.2005.03.025

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters (prfAP1, prfAP2, and PplcA). The prfAP2 promoter was previously proposed to be sigma B (sigma(B))-dependent. This hypothesis was tested by creating prfA promoter-gus transcriptional fusions in both L. monocytogenes wild-type (wt) and Delta sigB backgrounds and then measuring (i) beta-glucuronidase (GUS) activities; (ii) gus mRNA transcript levels; and (iii) the presence or absence of GUS in cells by immunofluorescence staining. prfAP2-directed expression increased as the wt L. monocytogenes strain entered stationary phase, whereas prfAP2-directed expression was greatly reduced in the Delta sigB strain, confirming both growth phase- and sigma(B)-dependent transcription of prfAP2. We conclude that prfAP2 is directly regulated by sigma(B). (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：329 / 336

页数：8

共 31 条

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