Self-assembling short oligopeptides and the promotion of angiogenesis

被引:151
作者
Narmoneva, DA
Oni, O
Sieminski, AL
Zhang, SG
Gertler, JP
Kamm, RD
Lee, RT
机构
[1] Harvard Univ, Sch Med, Boston, MA 02139 USA
[2] Brigham & Womens Hosp, Div Cardiovasc, Boston, MA 02139 USA
[3] MIT, Div Biol Engn, Cambridge, MA 02139 USA
[4] Massachusetts Gen Hosp, Div Vasc Surg, Vasc Surg Res Lab, Boston, MA 02114 USA
[5] Harvard Univ, Sch Med, Boston, MA 02114 USA
关键词
angiogenesis; endothelial cell; peptide; scaffold;
D O I
10.1016/j.biomaterials.2005.01.005
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Because an adequate blood supply to and within tissues is an essential factor for successful tissue regeneration, promoting a functional microvasculature is a crucial factor for biomaterials. In this study, we demonstrate that short self-assembling peptides form scaffolds that provide an angiogenic environment promoting long-term cell survival and capillary-like network formation in three-dimensional cultures of human microvascular endothelial cells. Our data show that, in contrast to collagen type I, the peptide scaffold inhibits endothelial cell apoptosis in the absence of added angiogenic factors, accompanied by enhanced gene expression of the angiogenic factor VEGF. In addition, our results suggest that the process of capillary-like network formation and the size and spatial organization of cell networks may be controlled through manipulation of the scaffold properties, with a more rigid scaffold promoting extended structures with a larger inter-structure distance, as compared with more dense structures of smaller size observed in a more compliant scaffold. These findings indicate that self-assembling peptide scaffolds have potential for engineering vascularized tissues with control over angiogenic processes. Since these peptides can be modified in many ways, they may be uniquely valuable in regeneration of vascularized tissues. (C) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4837 / 4846
页数:10
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