PR domain of Rous sarcoma virus Gag causes an assembly/budding defect in insect cells

被引：19

作者：

Johnson, MC ^{[1
]}

Scobie, HM ^{[1
]}

Vogt, VM ^{[1
]}

机构：

[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

来源：

JOURNAL OF VIROLOGY | 2001年 / 75卷 / 09期

关键词：

D O I：

10.1128/JVI.75.9.4407-4412.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

While baculovirus expression of Gag proteins from numerous retroviruses has led reliably to production of virus-like particles (VLPs), we observed that expression of Rous sarcoma virus Gag failed to produce VLPs. Transmission and scanning electron microscopy analysis revealed that the Gag protein reached the plasma membrane but was unable to correctly form particles. Addition of a myristylation signal had no effect on the budding defect, but deletion of the PR domain of Gag restored normal budding. The resulting VLPs were morphologically distinct from human immunodeficiency virus type 1 VLPs expressed in parallel.

引用

页码：4407 / 4412

页数：6

共 32 条

[1] Differential budding efficiencies of human T-cell leukemia virus type I (HTLV-I) Gag and Gag-Pro polyproteins from insect and mammalian cells [J].

Bouamr, F ;

Garnier, L ;

Rayne, F ;

Verna, A ;

Rebeyrotte, N ;

Cerutti, M ;

Mamoun, RZ .

VIROLOGY, 2000, 278 (02) :597-609

[2] In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: Identification of the p10 domain as a morphological determinant in the formation of spherical particles [J].

Campbell, S ;

Vogt, VM .

JOURNAL OF VIROLOGY, 1997, 71 (06) :4425-4435

[3] THE GAG PRECURSOR OF SIMIAN IMMUNODEFICIENCY VIRUS ASSEMBLES INTO VIRUS-LIKE PARTICLES [J].

DELCHAMBRE, M ;

GHEYSEN, D ;

THINES, D ;

THIRIART, C ;

JACOBS, E ;

VERDIN, E ;

HORTH, M ;

BURNY, A ;

BEX, F .

EMBO JOURNAL, 1989, 8 (09) :2653-2660

[4] Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle [J].

Fuller, SD ;

Wilk, T ;

Gowen, BE ;

Krausslich, HG ;

Vogt, VM .

CURRENT BIOLOGY, 1997, 7 (10) :729-738

[5] ASSEMBLY AND RELEASE OF HIV-1 PRECURSOR PR55GAG VIRUS-LIKE PARTICLES FROM RECOMBINANT BACULOVIRUS INFECTED INSECT CELLS [J].

GHEYSEN, D ;

JACOBS, E ;

DEFORESTA, F ;

THIRIART, C ;

FRANCOTTE, M ;

THINES, D ;

DEWILDE, M .

CELL, 1989, 59 (01) :103-112

[6] A conformational switch controlling HIV-1 morphogenesis [J].

Gross, I ;

Hohenberg, H ;

Wilk, T ;

Wiegers, K ;

Grättinger, M ;

Müller, B ;

Fuller, S ;

Kräusslich, HG .

EMBO JOURNAL, 2000, 19 (01) :103-113

[7] CLONING, BACTERIAL EXPRESSION, AND CHARACTERIZATION OF THE MASON-PFIZER MONKEY VIRUS PROTEINASE [J].

HRUSKOVAHEIDINGSFELDOVA, O ;

ANDREANSKY, M ;

FABRY, M ;

BLAHA, I ;

STROP, P ;

HUNTER, E .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (25) :15053-15058

[8] ENZYME-ACTIVITIES IN 4 DIFFERENT FORMS OF HUMAN IMMUNODEFICIENCY VIRUS-1 POL GENE-PRODUCTS [J].

HU, YW ;

KANG, CY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) :4596-4600

[9] Genetic determinants of Rous sarcoma virus particle size [J].

Krishna, NK ;

Campbell, S ;

Vogt, VM ;

Wills, JW .

JOURNAL OF VIROLOGY, 1998, 72 (01) :564-577

[10] THE BACULOVIRUS-INTEGRATED RETROTRANSPOSON TED ENCODES GAG AND POL PROTEINS THAT ASSEMBLE INTO VIRUS-LIKE PARTICLES WITH REVERSE-TRANSCRIPTASE [J].

LERCH, RA ;

FRIESEN, PD .

JOURNAL OF VIROLOGY, 1992, 66 (03) :1590-1601

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