Simultaneous detection of five coccidiostats in eggs by liquid chromatography-tandem mass spectrometry

被引:58
作者
Mortier, L
Daeseleire, E
Delahaut, P
机构
[1] Minist Flemish Community, Agr Res Ctr Ghent CLO, Dept Anim Prod Qual & Transformat Technol, B-9090 Melle, Belgium
[2] CER, Lab Hormonol, B-6900 Marloie, Belgium
关键词
coccidiostats; eggs; mass spectrometry;
D O I
10.1016/S0003-2670(02)01365-X
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A relatively fast, simple and very selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the detection of diclazuril, dimetridazole, halofuginone, nicarbazin and robenidine in eggs has been developed and validated. After a simple extraction with acetonitrile and evaporation of the organic phase, the extract was filtered and directly injected into the LC-MS-MS system on a C-18 column. The parent ions were selected for further fragmentation with argon. The developed method was validated according to Revision of Commission Decision 93/256/EC [EC, Final version of Commission Decision laying down analytical methods to be used for detecting substances and residues thereof in live animals and animal products according to Council Directive 96/23/EC (Revision of Commission Decision 93/256/EC)]. The validation parameters linearity, precision, recovery, specificity, decision limit (CCalpha) and detection capability (CCbeta) were determined. CCalpha varied from 0.75 mug kg(-1) for dimetridazole to 6 mug kg(-1) for diclazuril and CCbeta varied from 0.9 mug kg(-1) for dimetridazole up to 8.3 mug kg(-1) for diclazuril. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:27 / 37
页数:11
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