Evaluation of a Multiplex Real-Time PCR Assay for Detecting Pathogens in Cardiac Valve Tissue in Patients With Endocarditis

被引：21

作者：

Fernandez, Angel L. ^{[1
]}

Varela, Eduardo ^{[2
]}

Martinez, Lucia ^{[2
]}

Martinez, Amparo ^{[3
]}

Sierra, Juan ^{[1
]}

Gonzalez-Juanatey, Jose R. ^{[3
]}

Regueiro, Benito ^{[2
]}

机构：

[1] Hosp Clin Univ, Serv Cirugia Cardiaca, Santiago De Compostela 15706, A Coruna, Spain

[2] Hosp Clin Univ, Microbiol Serv, Santiago De Compostela 15706, A Coruna, Spain

[3] Hosp Clin Univ, Serv Cardiol, Santiago De Compostela 15706, A Coruna, Spain

来源：

REVISTA ESPANOLA DE CARDIOLOGIA | 2010年 / 63卷 / 10期

关键词：

Endocarditis; Polymerase chain reaction; INFECTIVE ENDOCARDITIS; MOLECULAR DIAGNOSIS; BACTERIAL;

D O I：

10.1016/S0300-8932(10)70254-2

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

With a novel real-time multiplex polymerase chain reaction test, the LightCycler SeptiFast (R) test, 25 bacterial and fungal species can be identified directly in blood. The SeptiFast (R) test has been used for rapid etiologic diagnosis in infectious endocarditis using blood samples but has not been evaluated directly on cardiac vegetations in patients being treated for infectious endocarditis. We prospectively analyzed 15 samples of heart valve tissue with active infectious endocarditis using the SeptiFast (R) test and compared the test's sensitivity with that of blood culture, valve tissue culture, and the SeptiFast (R) test in blood. The sensitivity of the SeptiFast test in heart valve tissue was 100%. The test results confirmed the diagnosis obtained using blood culture in 13 cases and identified the pathogen in 2 cases where blood culture tested negative. The sensitivity of the SeptiFast (R) test in heart valve tissue was greater than that obtained with conventional culture of vegetations or with the SeptiFast test in blood.

引用

页码：1205 / 1208

页数：4

共 14 条

[1] Impact of a molecular approach to improve the microbiological diagnosis of infective heart valve endocarditis [J].

Breitkopf, C ;

Hammel, D ;

Scheld, HH ;

Peters, G ;

Becker, K .

CIRCULATION, 2005, 111 (11) :1415-1421

[2] Evaluation of the LightCyclerA® SeptiFast test in the rapid etiologic diagnostic of infectious endocarditis [J].

Casalta, J. P. ;

Gouriet, F. ;

Roux, V. ;

Thuny, F. ;

Habib, G. ;

Raoult, D. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2009, 28 (06) :569-573

[3]

HABIB G, 2009, EUR HEART J

[4] Aetiological diagnosis of infective endocarditis by direct amplification of rRNA genes from surgically removed valve tissue. An 11-year experience in a Finnish teaching hospital [J].

Kotilainen, P ;

Heiro, M ;

Jalava, J ;

Rantakokko, V ;

Nikoskelainen, J ;

Nikkari, S ;

Rantakokko-Jalava, K .

ANNALS OF MEDICINE, 2006, 38 (04) :263-273

[5] Evaluation of PCR in the molecular diagnosis of endocarditis [J].

Lang, S ;

Watkin, RW ;

Lambert, PA ;

Bonser, RS ;

Littler, WA ;

Elliott, TSJ .

JOURNAL OF INFECTION, 2004, 48 (03) :269-275

[6]

LEHMANN L, 2009, CRIT CARE MED

[7] A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples [J].

Lehmann, Lutz Eric ;

Hunfeld, Klaus-Peter ;

Emrich, Thomas ;

Haberhausen, Gerd ;

Wissing, Heimo ;

Hoeft, Andreas ;

Stueber, Frank .

MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2008, 197 (03) :313-324

[8] Proposed modifications to the Duke criteria for the diagnosis of infective endocarditis [J].

Li, JS ;

Sexton, DJ ;

Mick, N ;

Nettles, R ;

Fowler, VG ;

Ryan, T ;

Bashore, T ;

Corey, GR .

CLINICAL INFECTIOUS DISEASES, 2000, 30 (04) :633-638

[9] Molecular diagnosis of infective endocarditis by real-time broad-range polymerase chain reaction (PCR) and Sequencing directly from heart valve tissue [J].

Marin, Mercedes ;

Munoz, Patricia ;

Sanchez, Monica ;

del Rosal, Marina ;

Alcala, Luis ;

Rodriguez-Creixems, Marta ;

Bouza, Emilio .

MEDICINE, 2007, 86 (04) :195-202

[10] Heart valves should not be routinely cultured [J].

Munoz, Patricia ;

Bouza, Emilio ;

Marin, Mercedes ;

Alcala, Luis ;

Creixems, Marta Rodriguez ;

Valerio, Maricela ;

Pinto, Angel .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (09) :2897-2901

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