Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice

被引：117

作者：

Asano, Takayuki ^{[1
,2
]}

Hakata, Makoto ^{[2
]}

Nakamura, Hidemitsu ^{[2
]}

Aoki, Naohiro ^{[1
]}

Komatsu, Setsuko ^{[2
]}

Ichikawa, Hiroaki ^{[2
]}

Hirochika, Hirohiko ^{[2
]}

Ohsugi, Ryu ^{[1
]}

机构：

[1] Univ Tokyo, Dept Agr & Environm Biol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[2] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan

来源：

PLANT MOLECULAR BIOLOGY | 2011年 / 75卷 / 1-2期

基金：

日本学术振兴会;

关键词：

ABA; Calcium-dependent protein kinase; FOX hunting system; Rice (Oryza sativa L.); Salt stress; RESPONSIVE GENE-EXPRESSION; FOX HUNTING SYSTEM; ABSCISIC-ACID; SIGNAL-TRANSDUCTION; TRANSCRIPTION FACTORS; DROUGHT TOLERANCE; STRESS-TOLERANCE; ABIOTIC STRESS; ACTIVATION; COLD;

D O I：

10.1007/s11103-010-9717-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcium acts as a messenger in various signal transduction pathways in plants. Calcium-dependent protein kinases (CDPKs) play important roles in regulating downstream components in calcium signaling pathways. In rice, the CDPKs constitute a large multigene family consisting of 29 genes, but the biological functions and functional divergence or redundancy of most of these genes remain unclear. Using a mini-scale full-length cDNA overexpressor (FOX) gene hunting system, we generated 250 independent transgenic rice plants overexpressing individual rice CDPKs (CDPK FOX-rice lines). These CDPK FOX-rice lines were screened for salt stress tolerance. The survival rate of the OsCPK21-FOX plants was higher than that of wild-type (WT) plants grown under high salinity conditions. The inhibition of seedling growth by abscisic acid (ABA) treatment was greater in the OsCPK21-FOX plants than in WT plants. Several ABA- and high salinity-inducible genes were more highly expressed in the OsCPK21-FOX plants than in WT plants. These results suggest that OsCPK21 is involved in the positive regulation of the signaling pathways that are involved in the response to ABA and salt stress.

引用

页码：179 / 191

页数：13

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