Proteins in action monitored by time-resolved FTIR spectroscopy

被引：118

作者：

Kötting, C ^{[1
]}

Gerwert, K ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Lehrstuhl Biophys, D-44801 Bochum, Germany

来源：

CHEMPHYSCHEM | 2005年 / 6卷 / 05期

关键词：

IR spectroscopy; isotopic labeling; proteins; reaction center; time-resolved spectroscopy;

D O I：

10.1002/cphc.200400504

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In the post genome era proteins coming into the focus of life sciences, X-ray structure analysis and NMR spectroscopy are established methods to determine the geometry of proteins. In order to determine the molecular reaction mechanism of proteins, time-resolved FTIR (trFTIR) difference spectroscopy emerges as a valuable tool. In this Minireview we describe the trFTIR difference spectroscopy and show its application on the light-driven proton pump bacteriorhodopsin (bR), the photosynthetic reaction center and the GTPase Ras, which is crucial in signal transduction. The main principles of the technique are presented, including a summary of triggering techniques, scan modes and analysis.

引用

页码：881 / 888

页数：8

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