Capillary electrophoresis of anions at high salt concentrations

被引:53
作者
Ding, WL
Thornton, MJ
Fritz, JS
机构
[1] Iowa State Univ, Ames Lab, Ames, IA 50011 USA
[2] Iowa State Univ, Dept Chem, Ames, IA 50011 USA
关键词
sea water; capillary electrophoresis; high salt concentrations; anion analysis; joule heating;
D O I
10.1002/elps.1150191216
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
It is commonly thought that even a moderately high ionic concentration in the background electrolyte (BGE) would leaad to Joule heating and serious peak distortion. However, we obtained very satisfactory separations of both inorganic and organic anions in electrolyte solutions as high as 5 M sodium chloride using direct photometric detection. Samples containing a 0.5 M concentration of a salt can be analyzed directly by making the BGE concentration of the same salt even higher to obtain electrostacking. The temperature in the center of the capillary was calculated to be 49 degrees C when the current is at its maximum of 280 mu A. The effect of various salts on electrophoretic and electroosmotic mobility is discussed. Several examples are given of capillary electrophoresis under high-salt conditions.
引用
收藏
页码:2133 / 2139
页数:7
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