Sensitivity of second harmonic generation from styryl dyes to transmembrane potential

被引:62
作者
Millard, AC
Jin, L
Wei, MD
Wuskell, JP
Lewis, A
Loew, LM
机构
[1] Univ Connecticut, Ctr Hlth, Ctr Biomed Imaging Technol, Farmington, CT 06030 USA
[2] Univ Connecticut, Ctr Hlth, Dept Physiol, Farmington, CT 06030 USA
[3] Hebrew Univ Jerusalem, Div Appl Phys, Jerusalem, Israel
关键词
D O I
10.1016/S0006-3495(04)74191-0
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In this article we present results from the simultaneous nonlinear (second harmonic generation and two-photon excitation fluorescence) imaging and voltage clamping of living cells. Specifically, we determine the sensitivity to transmembrane potential of second harmonic generation by ANEP-chromophore styryl dyes as a function of excitation wavelength and dye structure. We have measured second harmonic sensitivities of up to 43% per 100 mV, more than a factor of four better than the nominal voltage sensitivity of the dyes under "one-photon'' fluorescence. We find a dependence of voltage sensitivity on excitation wavelength that is consistent with a two-photon resonance, and there is a significant dependence of voltage sensitivity on the structure of the nonchromophore portion of the dyes.
引用
收藏
页码:1169 / 1176
页数:8
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