Production of vanillin by metabolically engineered Escherichia coli

被引：68

作者：

Yoon, SH

Li, C

Kim, JE

Lee, SH

Yoon, JY

Choi, MS

Seo, WT

Yang, JK

Kim, JY

Kim, SW ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Dept Food Sci & Nutr, Jinju 660701, South Korea

[2] Gyeongsang Natl Univ, Div Appl Life Sci BK21, Jinju 660701, South Korea

[3] Gyeongsang Natl Univ, Div Forest Sci, Jinju 660701, South Korea

[4] Jinju Natl Univ, Dept Food Sci, Jinju 660758, South Korea

[5] Gyeongsang Natl Univ, Environm Biotechnol Natl Core Res Ctr, Jinju 660701, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 22期

关键词：

ferulic acid; metabolic engineering; recombinant E. coli; vanillin;

D O I：

10.1007/s10529-005-3561-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

E. coli was metabolically engineered to produce vanillin by expression of the fcs and ech genes from Amycolatopsis sp. encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively. Vanillin production was optimized by leaky expression of the genes, under the IPTG-inducible trc promoter, in complex 2YT medium. Supplementation with glucose, fructose, galactose, arabinose or glycerol severely decreased vanillin production. The highest vanillin production of 1.1 g l(-1) was obtained with cultivation for 48 h in 2YT medium with 0.2% (w/v) ferulate, without IPTG and no supplementation of carbon sources.

引用

页码：1829 / 1832

页数：4

共 12 条

[1]

Identification of Amycolatopsis sp strain HR167 genes, involved in the bioconversion of ferulic acid to vanillin [J].