Stabilization of gas-phase noncovalent macromolecular complexes in electrospray mass spectrometry using aqueous triethylammonium bicarbonate buffer

被引:76
作者
Lemaire, D [1 ]
Marie, G [1 ]
Serani, L [1 ]
Laprévote, O [1 ]
机构
[1] CNRS, Inst Chim Subst Nat, Ctr Spectrometrie Masse, F-91198 Gif Sur Yvette, France
关键词
D O I
10.1021/ac001276s
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The use of triethylammonium bicarbonate (TEAB) solution in electrospray mass spectrometry proved to be avery efficient way for studying proteins or noncovalent protein complexes under "nondenaturing" conditions. The low charge states observed in the mass spectra improve the separation of ions arising from macromolecular species of close masses. Moreover, the multiply charged ions generated in a TEAB solution are significantly more stable than those formed under more conventional conditions (for example, with ammonium bicarbonate or acetate solution). re analytical interest of TEAB for the analysis of macromolecular species that can easily dissociate in the gas phase, such as hemoglobin or other macromolecular noncovalent complexes, is demonstrated.
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收藏
页码:1699 / 1706
页数:8
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