Identification of a maize endosperm-specific cDNA encoding farnesyl pyrophosphate synthetase

被引：32

作者：

Li, CP ^{[1
]}

Larkins, BA ^{[1
]}

机构：

[1] UNIV ARIZONA,DEPT PLANT SCI,TUCSON,AZ 85721

来源：

GENE | 1996年 / 171卷 / 02期

关键词：

prenyltransferase; gene expression; carotenoid;

D O I：

10.1016/0378-1119(95)00880-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Farnesyl pyrophosphate synthetase (FPS; EC 2.5.1.10) produces the 15-carbon farnesyl pyrophosphate which is utilized in the synthesis of sterols, carotenoids, dolichols, coenzyme Q, heme a and farnesylated proteins. We have cloned this mRNA sequence from a maize endosperm cDNA library and determined the 1378-nucleotide (nt) sequence of the DNA fragment. This sequence specifies an open reading frame of 1050 nt encoding FPS. The deduced amino acid sequence shows a high degree of similarity to FPS from a wide range of organisms. Southern blot analysis indicated that there are at least two FPS gene copies in the maize genome. The cloned FPS is expressed preferentially in maize endosperm and is up-regulated in the endosperm mutants, o2 and fl2.

引用

页码：193 / 196

页数：4

共 21 条

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