Engineering novel restriction endonucleases: Principles and applications

被引：39

作者：

Jeltsch, A

Wenz, C

Wende, W

Selent, U

Pingoud, A

机构：

来源：

TRENDS IN BIOTECHNOLOGY | 1996年 / 14卷 / 07期

关键词：

D O I：

10.1016/0167-7799(96)10030-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Restriction endonucleases cleave DNA with remarkable sequence specificity. In this review, we summarize the status of, and prospects for, engineering restriction endonucleases with new specificities. Such variants could be of considerable commercial value because restriction enzymes are among the most frequently used enzymes in molecular biology, and not all the desirable specificities are available. While it has not yet been possible to effect specificity changes, mutants have been described that (1) exhibit relaxed specificity, (2) favour modified substrates over their natural substrates, (3) discriminate between cleavage sites located in different sequences, (4) prefer metal ions other than Mg2+ as cofactors for cleavage, or (5) possess site-specific DNA-nicking activity.

引用

页码：235 / 238

页数：4

共 34 条

[1] CHANGING THE HYDROGEN-BONDING POTENTIAL IN THE DNA-BINDING SITE OF ECORI BY SITE-DIRECTED MUTAGENESIS DRASTICALLY REDUCES THE ENZYMATIC-ACTIVITY, NOT, HOWEVER, THE PREFERENCE OF THIS RESTRICTION ENDONUCLEASE FOR CLEAVAGE WITHIN THE SITE -GAATTC- [J].