Genomic sequence and structure of the human ABCG1 (ABC8) gene

被引:59
作者
Lorkowski, S
Rust, S
Engel, T
Jung, E
Tegelkamp, K
Galinski, EA
Assmann, G
Cullen, P
机构
[1] Univ Munster, Inst Arterioskleroseforsch, D-4400 Munster, Germany
[2] Univ Munster, Inst Biochem, D-4400 Munster, Germany
[3] Univ Munster, Inst Klin Chem, D-4400 Munster, Germany
[4] Univ Munster, Lab Med, D-4400 Munster, Germany
关键词
5 '-RACE; ATP-binding cassette transporter G1; ABC8; ABCG1; Drosophila white gene; promoter region; genomic structure; macrophages;
D O I
10.1006/bbrc.2000.4089
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The human ATP-binding cassette half transporter G1 (hABCG1) may play a role in cholesterol transport in macrophages, Using RACE assays we determined the structure of this gene. The hABCG1 gene spans more than 97 kb comprising 20 exons, 20 kb and 5 exons more than hitherto described. Four of the novel exons are upstream and one is downstream of previous exon 1, and they are predicted to encode at least five novel transcripts. We also detected two separate promoters, upstream of exons 1 and 5, respectively. The region 650 bp upstream of exon I was predicted to contain putative binding sites for SP1 and nuclear factor kappaB (NF-kappaB), but no sterol response elements (SREs) or retinoid X receptor (RXR) binding sites. The region 650 bp upstream of exon 5 contained 19 possible SP1 binding sites, one possible SRE, two possible NF-kappaB, and two putative RXR binding sites. Nevertheless, both promoters responded in macrophages to stimulation by hydroxy-cholesterol and retinoic acid. (C) 2001 Academic Press.
引用
收藏
页码:121 / 131
页数:11
相关论文
共 42 条
[1]   Characterization of the human ABC superfamily: Isolation and mapping of 21 new genes using the expressed sequence tags database [J].
Allikmets, R ;
Gerrard, B ;
Hutchinson, A ;
Dean, M .
HUMAN MOLECULAR GENETICS, 1996, 5 (10) :1649-1655
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   BACTERIAL PERIPLASMIC PERMEASES BELONG TO A FAMILY OF TRANSPORT PROTEINS OPERATING FROM ESCHERICHIA-COLI TO HUMAN - TRAFFIC ATPASES [J].
AMES, GF ;
MIMURA, CS ;
SHYAMALA, V .
FEMS MICROBIOLOGY LETTERS, 1990, 75 (04) :429-446
[4]  
[Anonymous], [No title captured]
[5]  
ASSMANN G, 1995, METABOLIC MOL BASIS, P1981
[6]  
Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkp985, 10.1093/nar/gkh121, 10.1093/nar/gkr1065]
[7]   The gene encoding ATP-binding cassette transporter 1 is mutated in Tangier disease [J].
Bodzioch, M ;
Orsó, E ;
Klucken, T ;
Langmann, T ;
Böttcher, L ;
Diederich, W ;
Drobnik, W ;
Barlage, S ;
Büchler, C ;
Porsch-Özcürümez, M ;
Kaminski, WE ;
Hahmann, HW ;
Oette, K ;
Rothe, G ;
Aslanidis, C ;
Lackner, KJ ;
Schmitz, G .
NATURE GENETICS, 1999, 22 (04) :347-351
[8]  
BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025
[9]   Mutations in ABC1 in Tangier disease and familial high-density lipoprotein deficiency [J].
Brooks-Wilson, A ;
Marcil, M ;
Clee, SM ;
Zhang, LH ;
Roomp, K ;
van Dam, M ;
Yu, L ;
Brewer, C ;
Collins, JA ;
Molhuizen, HOF ;
Loubser, O ;
Ouelette, BFF ;
Fichter, K ;
Ashbourne-Excoffon, KJD ;
Sensen, CW ;
Scherer, S ;
Mott, S ;
Denis, M ;
Martindale, D ;
Frohlich, J ;
Morgan, K ;
Koop, B ;
Pimstone, S ;
Kastelein, JJP ;
Genest, J ;
Hayden, MR .
NATURE GENETICS, 1999, 22 (04) :336-345
[10]   WEIGHT MATRIX DESCRIPTIONS OF 4 EUKARYOTIC RNA POLYMERASE-II PROMOTER ELEMENTS DERIVED FROM 502 UNRELATED PROMOTER SEQUENCES [J].
BUCHER, P .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 212 (04) :563-578