The effect of galE gene inactivation on lipopolysaccharide profile of Helicobacter pylori

被引:25
作者
Kwon, DH
Woo, JS
Perng, CL
Go, MF
Graham, DY
El-Zaatari, FAK [1 ]
机构
[1] Baylor Coll Med, Dept Med, Houston, TX 77030 USA
[2] Baylor Coll Med, Div Mol Virol, Houston, TX 77030 USA
[3] Vet Affairs Med Ctr, Digest Dis Res Lab, Houston, TX 77030 USA
关键词
D O I
10.1007/s002849900354
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The galE gene product, UDP-galactose 4-epimerase, mediates the incorporation of galactose in extracellular polysaccharide materials such as the O-side chain of lipopolysaccharide (LPS). The O-side chain in H. pylori LPS has been shown to cross-react with Lewis x and/or y blood group antigens, suggesting its potential involvement: in H. pylori-linked autoimmune disease. To study its role in H. pylori LPS biosynthesis, the galE gene was cloned. sequenced, and a galE-knockout H. pylori strain was constructed. The H. pylori galE gene encoded a protein of 344 amino acids with a molecular weight of 39K. The LPS profile from the galE-knockout H. pylori strain showed a lower molecular weight than that of the parental strain, indicating the involvement of the galE gene in LPS biosynthesis of H. pylori.
引用
收藏
页码:144 / 148
页数:5
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