The Escherichia coli CRISPR System Protects from λ Lysogenization, Lysogens, and Prophage Induction

被引:129
作者
Edgar, Rotem [1 ]
Qimron, Udi [1 ]
机构
[1] Tel Aviv Univ, Sackler Sch Med, Dept Clin Microbiol & Immunol, IL-69978 Tel Aviv, Israel
基金
以色列科学基金会;
关键词
PROVIDES ACQUIRED-RESISTANCE; INTERFERENCE; BACTERIA; ARCHAEA; PROKARYOTES; SEQUENCE; IMMUNITY; CLEAVAGE; DNA;
D O I
10.1128/JB.00644-10
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We show that phage lysogenization, lysogens, and prophage induction are all targeted by CRISPR. The results demonstrate that genomic DNA is not immune to the CRISPR system, that the CRISPR system does not require noncytoplasmic elements, and that the system protects from phages entering and exiting the lysogenic cycle.
引用
收藏
页码:6291 / 6294
页数:4
相关论文
共 20 条
[1]   Interference with histidyl-tRNA synthetase by a CRISPR spacer sequence as a factor in the evolution of Pelobacter carbinolicus [J].
Aklujkar, Muktak ;
Lovley, Derek R. .
BMC EVOLUTIONARY BIOLOGY, 2010, 10
[2]   Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants:: the Keio collection [J].
Baba, Tomoya ;
Ara, Takeshi ;
Hasegawa, Miki ;
Takai, Yuki ;
Okumura, Yoshiko ;
Baba, Miki ;
Datsenko, Kirill A. ;
Tomita, Masaru ;
Wanner, Barry L. ;
Mori, Hirotada .
MOLECULAR SYSTEMS BIOLOGY, 2006, 2 (1) :2006.0008
[3]   CRISPR provides acquired resistance against viruses in prokaryotes [J].
Barrangou, Rodolphe ;
Fremaux, Christophe ;
Deveau, Helene ;
Richards, Melissa ;
Boyaval, Patrick ;
Moineau, Sylvain ;
Romero, Dennis A. ;
Horvath, Philippe .
SCIENCE, 2007, 315 (5819) :1709-1712
[4]   Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin [J].
Bolotin, A ;
Ouinquis, B ;
Sorokin, A ;
Ehrlich, SD .
MICROBIOLOGY-SGM, 2005, 151 :2551-2561
[5]   Small CRISPR RNAs guide antiviral defense in prokaryotes [J].
Brouns, Stan J. J. ;
Jore, Matthijs M. ;
Lundgren, Magnus ;
Westra, Edze R. ;
Slijkhuis, Rik J. H. ;
Snijders, Ambrosius P. L. ;
Dickman, Mark J. ;
Makarova, Kira S. ;
Koonin, Eugene V. ;
van der Oost, John .
SCIENCE, 2008, 321 (5891) :960-964
[6]   Phage response to CRISPR-Encoded resistance in Streptococcus thermophilus [J].
Deveau, Helene ;
Barrangou, Rodolphe ;
Garneau, Josiane E. ;
Labonte, Jessica ;
Fremaux, Christophe ;
Boyaval, Patrick ;
Romero, Dennis A. ;
Horvath, Philippe ;
Moineau, Sylvain .
JOURNAL OF BACTERIOLOGY, 2008, 190 (04) :1390-1400
[7]   The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats [J].
Grissa, Ibtissem ;
Vergnaud, Gilles ;
Pourcel, Christine .
BMC BIOINFORMATICS, 2007, 8 (1)
[8]   RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex [J].
Hale, Caryn R. ;
Zhao, Peng ;
Olson, Sara ;
Duff, Michael O. ;
Graveley, Brenton R. ;
Wells, Lance ;
Terns, Rebecca M. ;
Terns, Michael P. .
CELL, 2009, 139 (05) :945-956
[9]   CRISPR/Cas, the Immune System of Bacteria and Archaea [J].
Horvath, Philippe ;
Barrangou, Rodolphe .
SCIENCE, 2010, 327 (5962) :167-170
[10]   Bacteriophage resistance mechanisms [J].
Labrie, Simon J. ;
Samson, Julie E. ;
Moineau, Sylvain .
NATURE REVIEWS MICROBIOLOGY, 2010, 8 (05) :317-327