A new image of plantain diversity assessed by SSR, AFLP and MSAP markers

被引:50
作者
Noyer, JL
Causse, S
Tomekpe, K
Bouet, A
Baurens, FC
机构
[1] CIRAD, UMR 1096 PIA, F-34398 Montpellier, France
[2] CIRAD, UMR 1098, BEPC, F-34398 Montpellier, France
[3] Ctr Africain Rech Banaiers & Plantains, CARBAP, Douala, Cameroon
关键词
DNA methylation; genetic diversity; Musa sp; polyploidy; vegetative multiplication;
D O I
10.1007/s10709-004-7319-z
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Using both SSR and AFLP markers, the genetic diversity of 30 plantains constituting a representative sample of the phenotypic diversity was assessed. The results confirmed a very narrow genetic base of this cultivar group. SSR and AFLP data support the hypothesis that these cultivars may have arisen from vegetative multiplication of a single seed. MSAP were used to survey cytosine methylation status at CCGG sites in order to obtain an alternative source of diversity data. A higher degree of polymorphism was revealed allowing the classification of the samples into three clusters. No correlation was observed between the phenotypic classification and methylation diversity. Implications for breeding programs are discussed.
引用
收藏
页码:61 / 69
页数:9
相关论文
共 23 条
[1]   Surveying CpG methylation at 5′-CCGG in the genomes of rice cultivars [J].
Ashikawa, I .
PLANT MOLECULAR BIOLOGY, 2001, 45 (01) :31-39
[2]   Using SD-AFLP and MSAP to assess CCGG methylation in the banana genome [J].
Baurens, FC ;
Bonnot, F ;
Bienvenu, D ;
Causse, S ;
Legavre, T .
PLANT MOLECULAR BIOLOGY REPORTER, 2003, 21 (04) :339-348
[3]   EVALUATION OF THE GENETIC DIVERSITY IN DIPLOID BANANAS (MUSA SPP) [J].
CARREEL, F ;
FAURE, S ;
DELEON, DG ;
LAGODA, PJL ;
PERRIER, X ;
BAKRY, F ;
DUMONTCEL, HT ;
LANAUD, C ;
HORRY, JP .
GENETICS SELECTION EVOLUTION, 1994, 26 :S125-S136
[4]   Analysis of DNA methylation in Arabidopsis thaliana based on methylation-sensitive AFLP markers [J].
Cervera, MT ;
Ruiz-García, L ;
Martínez-Zapater, JM .
MOLECULAR GENETICS AND GENOMICS, 2002, 268 (04) :543-552
[5]   Genetic characterization of banana cultivars (Musa spp.) from Brazil using microsatellite markers [J].
Creste, S ;
Neto, AT ;
Silva, SD ;
Figueira, A .
EUPHYTICA, 2003, 132 (03) :259-268
[6]   Comparative analysis of phenotypic and genotypic diversity among plantain landraces (Musa spp., AAB group) [J].
Crouch, HK ;
Crouch, JH ;
Madsen, S ;
Vuylsteke, DR ;
Ortiz, R .
THEORETICAL AND APPLIED GENETICS, 2000, 101 (07) :1056-1065
[7]  
Daniells J., 2001, Musalogue: a catalogue of Musa germplasm. Diversity in the genus Musa
[8]  
DUMONTCEL HT, 1983, FRUITS, V38, P461
[9]  
Finnegan E. J., 1996, Epigenetic mechanisms of gene regulation., P127
[10]   DNA methylation: A profile of methods and applications [J].
Fraga, ME ;
Esteller, M .
BIOTECHNIQUES, 2002, 33 (03) :632-+