High-density mapping of single-molecule trajectories with photoactivated localization microscopy

被引：583

作者：

Manley, Suliana ^{[1
]}

Gillette, Jennifer M. ^{[1
]}

Patterson, George H. ^{[1
]}

Shroff, Hari ^{[2
]}

Hess, Harald F. ^{[2
]}

Betzig, Eric ^{[2
]}

Lippincott-Schwartz, Jennifer ^{[1
]}

机构：

[1] NICHHD, NIH, Bethesda, MD 20892 USA

[2] Howard Hughes Med Inst, Ashburn, VA 20147 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

关键词：

D O I：

10.1038/NMETH.1176

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.

引用

页码：155 / 157

页数：3

共 14 条

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