Rac1 is required for the formation of three germ layers during gastrulation

被引：261

作者：

Sugihara, K

Nakatsuji, N

Nakamura, K

Nakao, K

Hashimoto, R

Otani, H

Sakagami, H

Kondo, H

Nozawa, S

Aiba, A

Katsuki, M

机构：

[1] Univ Tokyo, Inst Med Sci, Ctr Med Expt, Div DNA Biol & Embryo Engn,Minato Ku, Tokyo 1088639, Japan

[2] Univ Tokyo, CREST, Minato Ku, Tokyo 1088639, Japan

[3] Keio Univ, Sch Med, Dept Obstet & Gynecol, Shinjuku Ku, Tokyo 1608582, Japan

[4] Natl Inst Genet, Mammalian Dev Lab, Mishima, Shizuoka 4118540, Japan

[5] Shimane Med Univ, Dept Anat, Izumo, Shimane 6938501, Japan

[6] Tohoku Univ, Grad Sch Med Sci, Dept Cell Biol, Div Histol,Aoba Ku, Sendai, Miyagi 9808575, Japan

来源：

ONCOGENE | 1998年 / 17卷 / 26期

关键词：

Rac1; gene targeting; cell adhesion; motility; early development;

D O I：

10.1038/sj.onc.1202595

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Rad, a member of the Rho family proteins, regulates actin organization of cytoskeleton and cell adhesion. We used genetic analysis to elucidate the role of Rad in mouse embryonic development. The rac1 deficient embryos showed numerous cell deaths in the space between the embryonic ectoderm and endoderm at the primitive streak stage. Investigation of the primary epiblast culture isolated from rac1 deficient embryos indicated that Rad is involved in lamellipodia formation, cell adhesion and cell migration in vivo. These results suggest that Rad-mediated cell adhesion is essential for the formation of three germ layers during gastrulation.

引用

页码：3427 / 3433

页数：7

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