Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development

被引：10

作者：

Hu, Jie-Bi ^{[1
]}

Chen, Ting-Ru ^{[1
]}

Chen, Yu-Chie ^{[1
,2
]}

Urban, Pawel L. ^{[1
,2
]}

机构：

[1] Natl Chiao Tung Univ, Dept Appl Chem, Hsinchu 300, Taiwan

[2] Natl Chiao Tung Univ, Inst Mol Sci, Hsinchu 300, Taiwan

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

FIREFLY LUCIFERASE;

D O I：

10.1038/srep08135

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 mu M. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.

引用

页数：5

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