Identifying proteins and post-translational modifications by mass spectrometry

被引:86
作者
Küster, B [1 ]
Mann, M [1 ]
机构
[1] Odense Univ, Ctr Expt Bioinformat, DK-5230 Odense M, Denmark
关键词
D O I
10.1016/S0959-440X(98)80075-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Major recent advances in hardware performance, sample-handling procedures and software algorithms now allow reliable and sensitive mass spectrometric identification of proteins. Mass spectrometry vastly outperforms traditional sequencing technologies and thereby greatly facilitates the elucidation of the functions of individual proteins as well as multiprotein complexes and larger protein assemblages.
引用
收藏
页码:393 / 400
页数:8
相关论文
共 67 条
[1]   The essential role of mass spectrometry in characterizing protein structure: Mapping posttranslational modifications [J].
Annan, RS ;
Carr, SA .
JOURNAL OF PROTEIN CHEMISTRY, 1997, 16 (05) :391-402
[2]  
Bahr U, 1997, J MASS SPECTROM, V32, P1111, DOI 10.1002/(SICI)1096-9888(199711)32:10<1111::AID-JMS567>3.0.CO
[3]  
2-Y
[4]   Identification of phosphorylation sites on neurofilament proteins by nanoelectrospray mass spectrometry [J].
Betts, JC ;
Blackstock, WP ;
Ward, MA ;
Anderton, BH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (20) :12922-12927
[5]   MASS RESOLUTION IMPROVEMENT BY INCORPORATION OF PULSED ION EXTRACTION IN A MATRIX-ASSISTED LASER-DESORPTION IONIZATION LINEAR TIME-OF-FLIGHT MASS-SPECTROMETER [J].
BROWN, RS ;
LENNON, JJ .
ANALYTICAL CHEMISTRY, 1995, 67 (13) :1998-2003
[6]   Mass spectrometry of whole proteins eluted from sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels [J].
Cohen, SL ;
Chait, BT .
ANALYTICAL BIOCHEMISTRY, 1997, 247 (02) :257-267
[7]   Rapid protein sequencing by tandem mass spectrometry and cDNA cloning of p20-CGGBP - A novel protein that binds to the unstable triplet repeat 5'-d(CGG)(n)-3' in the human FMR1 gene [J].
Deissler, H ;
Wilm, M ;
Genc, B ;
Schmitz, B ;
Ternes, T ;
Naumann, F ;
Mann, M ;
Doerfler, W .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (27) :16761-16768
[8]   Emerging tandem-mass-spectrometry techniques for the rapid identification of proteins [J].
Dongre, AR ;
Eng, JK ;
Yates, JR .
TRENDS IN BIOTECHNOLOGY, 1997, 15 (10) :418-425
[9]   Analysis of proteins by direct scanning infrared-MALDI mass spectrometry after PD PAGE separation and electroblotting [J].
Eckerskorn, C ;
Strupat, K ;
Schleuder, D ;
Hochstrasser, D ;
Sanchez, JC ;
Lottspeich, F ;
Hillenkamp, F .
ANALYTICAL CHEMISTRY, 1997, 69 (15) :2888-2892
[10]   AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE [J].
ENG, JK ;
MCCORMACK, AL ;
YATES, JR .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) :976-989