Cyclic luciferase for real-time sensing of caspase-3 activities in living mammals

被引：109

作者：

Kanno, Akira

Yamanaka, Yuko

Hirano, Hisashi

Umezawa, Yoshio

Ozawa, Takeaki

机构：

[1] Inst Mol Sci, PRESTO, Dept Mol Struct, Japan Sci & Technol Agcy, Okazaki, Aichi 444, Japan

[2] Univ Tokyo, Sch Sci, Dept Chem, Japan Sci & Technol Agcy, Tokyo 1130033, Japan

[3] Yokohama City Univ, Int Grad Sch Arts & Sci, Supramol Biol, Yokohama, Kanagawa 2440813, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 40期

关键词：

apoptosis; caspases; imaging; luciferases; luminescence;

D O I：

10.1002/anie.200700538

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

© 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. (Figure Presented) Illumination for apoptosis: Firefly luciferase connected with a substrate sequence for caspase-3 (DEVD) is cyclized by an intein DnaE. When the cyclic luciferase is expressed in living cells, its activity is greatly decreased because of a steric effect. Activated caspase-3 cleaves the substrate sequence in the cyclic luciferase and the luciferase activity is restored. Quantitative sensing of time-dependent caspase-3 activity in living cells and mice upon extracellular stimuli has been demonstrated (see images).

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收藏

页码：7595 / 7599

页数：5

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