Ligand-induced formation of triple helices with antiparallel third strands containing G and T

被引:28
作者
Escude, C
Sun, JS
Nguyen, CH
Bisagni, E
Garestier, T
Helene, C
机构
[1] MUSEUM NATL HIST NAT, BIOPHYS LAB, CNRS URA 481, INSERM U201, F-75231 PARIS 05, FRANCE
[2] INST CURIE, SYNTH ORGAN LAB, CNRS URA 1387, F-91405 ORSAY, FRANCE
关键词
D O I
10.1021/bi960120c
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have examined the effects of benzopyridoindole derivatives on triple helices with antiparallel third strands. Absorption spectroscopy, footprinting, and gel retardation experiments demonstrate that a benzopyridoindole derivative (BePI) is able to induce formation of a triple helix with an antiparallel (G,T)-containing third strand, which does not form in the absence of this ligand. This triple-helical complex is very stable with a half-dissociation temperature as high as 51 degrees C, and its formation is pH independent. Antiparallel oligonucleotides containing thymine and guanine bind strongly to double-helical DNA under physiological conditions in the presence of only 0.5 mu M BePI. Formation of a BePI-stabilized triple helix strongly inhibits cleavage of the target duplex by DNase I.
引用
收藏
页码:5735 / 5740
页数:6
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