Reconstruction of cartilage, bone, and hematopoietic microenvironment with demineralized bone matrix and bone marrow cells

被引：43

作者：

Gurevitch, O ^{[1
]}

Kurkalli, BGS ^{[1
]}

Prigozhina, T ^{[1
]}

Kasir, J ^{[1
]}

Gaft, A ^{[1
]}

Slavin, S ^{[1
]}

机构：

[1] Hadassah Univ Hosp, Dept Bone Marrow Transplant & Canc Immunotherapy, Cell Therapy & Transplant Biol Res Ctr, IL-91120 Jerusalem, Israel

来源：

STEM CELLS | 2003年 / 21卷 / 05期

关键词：

bone; cartilage; stromal microenvironment; bone marrow; demineralized bone matrix; reconstruction;

D O I：

10.1634/stemcells.21-5-588

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Highly specialized hard tissues, such as cartilage, bone, and stromal microenvironment supporting hematopoiesis, originate from a common type of mesenchymal progenitor cell (MPC). We hypothesized that MPCs present in bone marrow cell suspension and demineralized bone matrix (DBM) that possess natural conductive and inductive features might constitute a unit containing all the essential elements for purposive bone and cartilage induction. Using a rodent preclinical model, we found that implantation of a composite comprising DBM and MPCs into A) a damaged area of a joint; B) an ablated bone marrow cavity, and C) a calvarial defect resulted in the generation of A) a new osteochondral complex comprising articular cartilage and subchondral bone; B) trabecular bone and stromal microenvironment supporting hematopoiesis, and C) flat bone, respectively. The new tissue formation followed differentiation pathways controlled by site-specific physiological conditions, thus developing tissues that precisely met local demands.

引用

页码：588 / 597

页数：10

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