Three-dimensional structure of the anaphase-promoting complex

被引：61

作者：

Gieffers, C

Dube, P

Harris, JR

Stark, H

Peters, JM

机构：

[1] Res Inst Mol Pathol, A-1030 Vienna, Austria

[2] Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany

[3] Johannes Gutenberg Univ Mainz, Inst Zool, D-55099 Mainz, Germany

来源：

MOLECULAR CELL | 2001年 / 7卷 / 04期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1016/S1097-2765(01)00234-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The anaphase-promoting complex (APC) is a cell cycle-regulated ubiquitin-protein ligase, composed of at least 11 subunits, that controls progression through mitosis and G1. Using cryo-electron microscopy and angular reconstitution, we have obtained a three-dimensional model of the human APC at a resolution of 24 Angstrom. The APC has a complex asymmetric structure 140 Angstrom x 140 Angstrom x 135 Angstrom in size, in which an outer protein wall surrounds a large inner cavity. We discuss the possibility that this cavity represents a reaction chamber in which ubiquitination reactions take place, analogous to the inner cavities formed by other protein machines such as the 26S proteasome and chaperone complexes. This cage hypothesis could help to explain the great subunit complexity of the APC.

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页码：907 / 913

页数：7

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