Hydraulic characteristics of aerobic granules using size exclusion chromatography

被引:14
作者
Adav, Sunil S. [1 ]
Chang, Cheng-Hsuan [1 ]
Lee, Duu-Jong [1 ]
机构
[1] Natl Taiwan Univ, Dept Chem Engn, Taipei 10617, Taiwan
关键词
aerobic granules; size exclusion chromatography; pore size; permeability; dispersion; models;
D O I
10.1002/bit.21656
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Elution of poly(ethylene glycol) of molecular weight 200-20,000 Da from a size exclusion chromatography column packed with phenol-fed aerobic granules of three different nominal sizes (types I-III) has been investigated. The pore sizes of the three types of granules were evaluated based on the mean hydraulic times of the elution curves that decreased directly proportional to the increased logarithm of the molecular mass of a standard tracer and increased as granule size decreased. The corresponding exclusion limits for types I-III granules were 139,000, 123,000, and 54,500 Da, respectively. A one-dimensional convection-dispersion model described the effective dispersion coefficients of the tracers through the granule column. The intra-granular permeabilities and convective and diffusional transit times through the granule interior were evaluated by a dual porosity model. For small molecules of molecular mass < 5,000 Da, intra-granular convection dominated transport mechanisms at fast moving velocity. For comparatively larger molecules, diffusion barrier existed to limit nutrient supply to the granules. The size exclusion test provided intra granular transport characteristics using detailed analysis on the elution data.
引用
收藏
页码:791 / 799
页数:9
相关论文
共 22 条
[1]   Degradation of phenol by Acinetobacter strain isolated from aerobic granules [J].
Adav, Sunil S. ;
Chen, Ming-Yuan ;
Lee, Duu-Jong ;
Ren, Nan-Qi .
CHEMOSPHERE, 2007, 67 (08) :1566-1572
[2]   Degradation of phenol by aerobic granules and isolated yeast Candida tropicalis [J].
Adav, Sunil S. ;
Chen, Ming-Yuan ;
Lee, Duu-Jong ;
Ren, Nan-Qi .
BIOTECHNOLOGY AND BIOENGINEERING, 2007, 96 (05) :844-852
[3]   DETERMINATION OF THE PERMEABILITY AND POROSITY OF ANAEROBIC SLUDGE GRANULES BY SIZE EXCLUSION CHROMATOGRAPHY [J].
ALPHENAAR, PA ;
PEREZ, MC ;
VANBERKEL, WJH ;
LETTINGA, G .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1992, 36 (06) :795-799
[4]  
[Anonymous], STAND METH EX WAT WA
[5]   Aerobic granulation with industrial wastewater in sequencing batch reactors [J].
Arrojo, B ;
Mosquera-Corral, A ;
Garrido, JM ;
Méndez, R .
WATER RESEARCH, 2004, 38 (14-15) :3389-3399
[6]   Aerobic granulation in a sequencing batch reactor [J].
Beun, JJ ;
Hendriks, A ;
Van Loosdrecht, MCM ;
Morgenroth, E ;
Wilderer, PA ;
Heijnen, JJ .
WATER RESEARCH, 1999, 33 (10) :2283-2290
[7]   Distribution of extracellular polymeric substances in aerobic granules [J].
Chen, M. Y. ;
Lee, D. J. ;
Tay, J. H. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 73 (06) :1463-1469
[8]   Staining of extracellular polymeric substances and cells in bioaggregates [J].
Chen, Ming-Yuan ;
Lee, Duu-Jong ;
Tay, Joo-Hwa ;
Show, Kuan-Yeow .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 75 (02) :467-474
[9]   Oxygen diffusion in active layer of aerobic granule with step change in surrounding oxygen levels [J].
Chiu, Z. C. ;
Chen, M. Y. ;
Lee, D. J. ;
Wang, C. H. ;
Lai, J. Y. .
WATER RESEARCH, 2007, 41 (04) :884-892
[10]   Diffusivity of oxygen in aerobic granules [J].
Chiu, Z. C. ;
Chen, M. Y. ;
Lee, D. J. ;
Tay, S. T. -L. ;
Tay, J. H. ;
Show, K. Y. .
BIOTECHNOLOGY AND BIOENGINEERING, 2006, 94 (03) :505-513