Direct measurement of acetylcholine release in detrusor smooth muscles isolated from rabbits

被引:8
作者
Inadome, A [1 ]
Yoshida, M [1 ]
Takahashi, W [1 ]
Yono, M [1 ]
Seshita, H [1 ]
Miyamoto, Y [1 ]
Ueda, S [1 ]
机构
[1] Kumamoto Univ, Sch Med, Dept Urol, Kumamoto 8608556, Japan
来源
UROLOGICAL RESEARCH | 1998年 / 26卷 / 05期
关键词
acetylcholine; detrusor smooth muscle; microdialysis; high performance liquid chromatography; non-adrenergic non-cholinergic neurotransmitters; prejunctional receptor;
D O I
10.1007/s002400050062
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
In the present study, we measured acetylcholine (ACh) released from rabbit detrusor smooth muscle strips induced by electrical held stimulation (EFS) using high-performance liquid chromatography coupled with microdialysis procedure. There were frequency- and duration-dependent increases in contractile response and ACh release. There was a significant, but not simple correlation between EFS-induced contraction and ACh release. Atropine caused a decrease and increase in the contractile response and ACh release, respectively. Pretreatment with propranolol increased ACh release, but pretreatment with phentolamine had no significant effect. These results demonstrate that this method is applicable to direct measurement of ACh release by EFS, and that neurotransmitters other than ACh may relate to EFS-induced contraction. In addition, it is suggested that there are prejunctional inhibitory muscarinic receptors and beta-adrenoceptors, which contribute to ACh release induced by EFS in the rabbit detrusor smooth muscles.
引用
收藏
页码:311 / 317
页数:7
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