Detection and genotyping of human papillomavirus in cervical samples from Italian patients

被引:47
作者
De Francesco, MA
Gargiulo, F
Schreiber, C
Ciravolo, G
Salinaro, F
Mancal, N
机构
[1] Univ Brescia, Spedali Civili, Inst Microbiol & Virol, Brescia, Italy
[2] Univ Brescia, Spedali Civili, Dept Obstet & Ginecol, Brescia, Italy
关键词
PCR; HPV; sequence;
D O I
10.1002/jmv.20306
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human papillomaviruses (HPVs) are etiological agents of cervical cancer. In order to assess the epidemiological incidence and frequency of different HPV types, we applied a polymerase chain reaction (PCR)-direct sequencing approach based on the use of MY09/MY11 primers as compared to Hybrid Capture assay. Cervical samples were taken from 1,500 women, both with normal and abnormal cytological smears, and we found an incidence of 6.6% of HPV infection in Brescia. Overall, 97 samples tested HPV-positive, yielding 18 HPV types. The four most frequent HPV types were: HPV 16, -31, -6, and -58. This approach could be used in ordinary laboratory settings for quick and reliable typing of known and novel HPVs from clinical specimens and it could also be applied to anti-cancer vaccine development.
引用
收藏
页码:588 / 592
页数:5
相关论文
共 35 条
  • [1] PREVALENCE OF HUMAN PAPILLOMAVIRUS IN CERVICAL-CANCER - A WORLDWIDE PERSPECTIVE
    BOSCH, FX
    MANOS, MM
    MUNOZ, N
    SHERMAN, M
    JANSEN, AM
    PETO, J
    SCHIFFMAN, MH
    MORENO, V
    KURMAN, R
    SHAH, KV
    ALIHONOU, E
    BAYO, S
    MOKHTAR, HC
    CHICAREON, S
    DAUDT, A
    DELOSRIOS, E
    GHADIRIAN, P
    KITINYA, JN
    KOULIBALY, M
    NGELANGEL, C
    TINTORE, LMP
    RIOSDALENZ, JL
    SARJADI
    SCHNEIDER, A
    TAFUR, L
    TEYSSIE, AR
    ROLON, PA
    TORROELLA, M
    TAPIA, AV
    WABINGA, HR
    ZATONSKI, W
    SYLLA, B
    VIZCAINO, P
    MAGNIN, D
    KALDOR, J
    GREER, C
    WHEELER, C
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1995, 87 (11): : 796 - 802
  • [2] Determinants of cervical human papillomavirus infection: Differences between high- and low-oncogenic risk types
    Chan, PKS
    Chang, AR
    Cheung, JLK
    Chan, DPC
    Xu, LY
    Tang, NLS
    Cheng, AF
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2002, 185 (01) : 28 - 35
  • [3] HUMAN PAPILLOMAVIRUS INFECTION IS TRANSIENT IN YOUNG-WOMEN - A POPULATION-BASED COHORT STUDY
    EVANDER, M
    EDLUND, K
    GUSTAFSSON, A
    JONSSON, M
    KARLSSON, R
    RYLANDER, E
    WADELL, G
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (04) : 1026 - 1030
  • [4] Epidemiology of acquisition and clearance of cervical human papillomavirus infection in women from a high-risk area for cervical cancer
    Franco, EL
    Villa, LL
    Sobrinho, JP
    Prado, JM
    Rousseau, MC
    Désy, M
    Rohan, TE
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1999, 180 (05) : 1415 - 1423
  • [5] Evaluation of human papillomavirus consensus primers for HPV detection by the polymerase chain reaction
    Harnish, DG
    Belland, LM
    Scheid, EE
    Rohan, TE
    [J]. MOLECULAR AND CELLULAR PROBES, 1999, 13 (01) : 9 - 21
  • [6] Population-based study of human papillomavirus infection and cervical neoplasia in rural Costa Rica
    Herrero, R
    Hildesheim, A
    Bratti, C
    Sherman, ME
    Hutchinson, M
    Morales, J
    Balmaceda, I
    Greenberg, MD
    Alfaro, M
    Burk, RD
    Wacholder, S
    Plummer, M
    Schiffman, M
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2000, 92 (06): : 464 - 474
  • [7] PERSISTENT GENITAL HUMAN PAPILLOMAVIRUS INFECTION AS A RISK FACTOR FOR PERSISTENT CERVICAL DYSPLASIA
    HO, GYF
    BURK, RD
    KLEIN, S
    KADISH, AS
    CHANG, CJ
    PALAN, P
    BASU, J
    TACHEZY, R
    LEWIS, R
    ROMNEY, S
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1995, 87 (18): : 1365 - 1371
  • [8] Natural history of cervicovaginal papillomavirus infection in young women
    Ho, GYF
    Bierman, R
    Beardsley, L
    Chang, CJ
    Burk, RD
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1998, 338 (07) : 423 - 428
  • [9] Jacobs MV, 2000, INT J CANCER, V87, P221, DOI 10.1002/1097-0215(20000715)87:2&lt
  • [10] 221::AID-IJC11&gt