Solubility of artificial proteins with random sequences

被引：57

作者：

Prijambada, ID

Yomo, T

Tanaka, F

Kawama, T

Yamamoto, K

Hasegawa, A

Shima, Y

Negoro, S

Urabe, I

机构：

[1] OSAKA UNIV,FAC ENGN,DEPT BIOTECHNOL,SUITA,OSAKA 565,JAPAN

[2] NARA MED UNIV,DEPT CHEM,KASHIHARA,NARA 634,JAPAN

来源：

FEBS LETTERS | 1996年 / 382卷 / 1-2期

关键词：

artificial protein; enzyme evolution; protein solubility; random protein;

D O I：

10.1016/0014-5793(96)00123-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A library of artificial random proteins of 141 amino acid residues of which 95 are random and,which includes the 20 kinds of amino acids was prepared. Out of the 25 identified random proteins, 5 were soluble in the cell lysate, indicating that about 20% of the random proteins expressed in Escherichia coli are expected to be soluble. The soluble random proteins RP3-42 and RP3-45 and insoluble RP3-70 were purified. The solubility of the purified form is the same as that in the cell lysate.

引用

页码：21 / 25

页数：5

共 11 条

[1] CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION IN ESCHERICHIA-COLI OF DNA-POLYMERASE GENE (POLA) FROM THERMUS-THERMOPHILUS HB8 [J].