N-linked carbohydrate on human leukocyte antigen-C and recognition by natural killer cell inhibitory receptors

被引:14
作者
Baba, E [1 ]
Erskine, R [1 ]
Boyson, JE [1 ]
Cohen, GB [1 ]
Davis, DM [1 ]
Malik, P [1 ]
Mandelboim, O [1 ]
Reyburn, HT [1 ]
Strominger, JL [1 ]
机构
[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
关键词
MHC; NK cells; inhibitory receptor; carbohydrate;
D O I
10.1016/S0198-8859(00)00184-1
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The possible role of carbohydrate in the interaction of HLA-C with a human inhibitory natural Killer cell Immunoglobulin-like Receptor with two Ib domains, KIR2DL1, was investigated. Transfectants of 721.221 (a class I MHC-negative human B cell line) expressing only HLA-Cw4 or -Cw6 or their respective non-glycosylated mutants (N86Q, S88A) were made. The binding of a KIR2DL1-Ig fusion protein to the non-glycosylated mutant HLA-Cw4- or -Cw6-expressing cells was markedly decreased compared to the wild type-expressing cells. The ability to induce an inhibitory signal in the NK tumor line YTS transfected with KIR2DL1 was also impaired in the nonglycosylated mutant expressing cells. Furthermore, in a second functional assay, mutant HLA-Cw I and -Cw6 molecules had impaired ability to induce signal transduction in BW cells expressing a KIR2DL1-CD3 zeta chain chimeric protein. Thus, the deletion of the N-linked glycosylation signal in HLA-Cw4 and -Cw6 greatly reduced recognition by KIR2DL1. Alternative interpretations of the data are discussed. (C) American Society for Histocompatibility and Immunogenetics, 2000. Published by Elsevier Science Inc.
引用
收藏
页码:1202 / 1218
页数:17
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