Precise determination of RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli

被引：22

作者：

Thiede, B ^{[1
]}

Urlaub, H ^{[1
]}

Neubauer, H ^{[1
]}

Grelle, G ^{[1
]}

Wittmann-Liebold, B ^{[1
]}

机构：

[1] Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany

来源：

BIOCHEMICAL JOURNAL | 1998年 / 334卷

关键词：

D O I：

10.1042/bj3340039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA-protein cross-linked complexes were isolated and purified to obtain precise data about RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli. N-terminal microsequencing and matrix-assisted laser desorption ionization MS were used to identify the cross-linking sites at the amino acid and nucleotide levels. In this manner the following contact sites of five ribosomal proteins with the 23 S rRNA were established: Lys-67 of L2 to U-1963, Tyr-35 of L4 to U-615, Lys-97 of L21 to U-546, Lys-49 of L23 to U-139 or C-140 and Lys-71 and Lys-74 of L27 to U-2334.

引用

页码：39 / 42

页数：4

共 34 条

[1] THE BINDING-SITE FOR RIBOSOMAL PROTEIN-L2 WITHIN 23S RIBOSOMAL-RNA OF ESCHERICHIA-COLI [J].